Properties of mouse CD40: cellular distribution of CD40 and B cell activation by monoclonal anti-mouse CD40 antibodies

European Journal of Immunology
J HasboldG G Klaus

Abstract

We describe here the derivation of a rat monoclonal antibody (mAb) against mouse CD40 (designated 3/23), which stains 45-50% of spleen cells of adult mice, approximately 90% of which are B cells. Interestingly, some 5-10% of both CD4+ and CD8+ T cells in the spleens of (some, but not all) adult, unimmunized mice are also CD40+, whereas CD40+ cells were not detectable in the thymus, even following collagenase digestion. Some 35-40% of lymphoid cells in the bone marrow of adult mice are CD40+ and virtually all of these are B220+, and hence of the B cell lineage: triple-color flow cytometry showed that CD40 is expressed at low levels on some 30% of pre-B cells, at intermediate levels on 80% of immature B cells and on essentially all mature B cells in the bone marrow. These results, therefore, suggest that in the mouse CD40 is expressed relatively late during the process of B cell differentiation. The mAb induced marked up-regulation of major histocompatibility complex class II molecules, CD23 and B7.2 antigens on mature B cells. It also stimulated modest levels of DNA synthesis in mature B cells by itself: this was markedly enhanced by suboptimal concentrations of mitogenic (but not non-mitogenic) anti-mu and anti-delta mAb, and m...Continue Reading

References

Dec 1, 1992·European Journal of Immunology·D GrafR A Kroczek
May 7, 1992·Nature·R J ArmitageC R Maliszewski
Jul 1, 1990·Tissue Antigens·E A Clark
Feb 1, 1991·Scandinavian Journal of Immunology·P BjörckS Paulie
Dec 21, 1989·Nature·Y J LiuI C MacLennan
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E A Clark, J A Ledbetter
Jan 1, 1994·European Journal of Immunology·M K KennedyK H Grabstein
Apr 1, 1993·The Journal of Experimental Medicine·E A Ranheim, T J Kipps
May 1, 1993·European Journal of Immunology·K WheelerR Jefferis
Aug 1, 1993·The Journal of Experimental Medicine·M R AldersonM K Spriggs
Sep 1, 1993·European Journal of Immunology·R J ArmitageM R Alderson
Feb 3, 1994·Nature·E A Clark, J A Ledbetter
Sep 1, 1993·Current Biology : CB·J F Bazan

❮ Previous
Next ❯

Citations

Feb 1, 1995·Current Opinion in Immunology·P D Hodgkin, A Basten
Apr 26, 2002·Toxicology·Louis BoonMark de Boer
Nov 22, 1997·Current Opinion in Immunology·C P Larsen, T C Pearson
Apr 25, 2007·Immunology and Cell Biology·Philip D Hodgkin
Oct 9, 1998·Clinical and Experimental Immunology·T IwasakiE Kakishita
Jun 10, 1998·The Journal of Experimental Medicine·A MatsudaA Takeda
May 8, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Emily L WilliamsJuliet C Gray
Feb 19, 2011·Journal of Leukocyte Biology·Jennifer CarlringAndrew W Heath
Sep 16, 2008·European Journal of Immunology·Juliet C GrayMartin J Glennie
Aug 19, 2004·The Journal of Allergy and Clinical Immunology·David QuarcooEckard Hamelmann
Jul 19, 2012·International Reviews of Immunology·Alexander L RakhmilevichPaul M Sondel
Mar 31, 1998·Scandinavian Journal of Immunology·K AxcronaT Leanderson
May 9, 2001·Nature·A J McAdamA H Sharpe
Apr 14, 2000·International Reviews of Immunology·J L Press
Jan 7, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·L LefrançoisS Olson
Dec 20, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M P FischbeinB Bonavida
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·L D EricksonT J Waldschmidt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.