Abstract
Cancer procoagulant (CP) and tissue factor (TF; only in complex with Factor VIIa (FVIIa)) can activate FX to FXa. Controversy still exists whether or not CP is an entity different from TF, or whether CP activity is due to contamination of CP preparations with TF/FVIIa complex. We therefore looked for proteins in CP preparations that were detected by anti-TF antibodies and then sequenced these proteins. One- and two-dimensional gels of CP and TF were used to identify proteins immunoreactive to monoclonal anti-CP and anti-TF antibodies (Mabs). Those proteins in the CP preparation recognized by anti-TF antibodies were sequenced. Angiotensinogen precursor, alpha-1-antitrypsin precursor, and vitamin D-binding protein were identified along with one so far unidentified sequence; however, no TF-sequences were identified. Also, no proteins with the correct molecular weight for TF were identified using anti-TF antibodies. It seems possible that CP preparations contain proteins that have some epitopes similar to the epitopes recognized in TF by anti-TF Mab. However, these proteins do neither have the molecular weight nor the amino acid sequence of TF.
References
Sep 1, 1992·Cancer·N S CallanderL V Rao
Jan 1, 1992·Virchows Archiv. A, Pathological Anatomy and Histopathology·U SturmM Müller
Sep 15, 1991·The Biochemical Journal·W RufT S Edgington
Mar 15, 1989·Thrombosis Research·H TanakaT Maekawa
Nov 1, 1988·Thrombosis Research·J H MorrisseyT S Edgington
Dec 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·L CuratoloN Semeraro
May 15, 1988·Thrombosis Research·J H MorrisseyT S Edgington
Sep 24, 1985·Biochemistry·A Falanga, S G Gordon
Oct 14, 1995·Lancet·A K KakkarR C Williamson
Aug 1, 1995·The British Journal of Surgery·A K KakkarR C Williamson
Jan 1, 1993·Virchows Archiv. B, Cell Pathology Including Molecular Pathology·M MüllerY Zhang
Feb 1, 1996·Nature Medicine·J ContrinoF R Rickles
May 1, 1996·Cancer·K HamadaY Ushio
Dec 1, 1996·Thrombosis Research·A AmirkhosraviJ L Francis
Jul 15, 1997·Thrombosis Research·W P MielickiS G Gordon
Sep 1, 1996·Current Opinion in Hematology·W Ruf, B M Mueller
Mar 21, 1998·Journal of Molecular Biology·M HuangI A Wilson
Apr 29, 1998·Cancer·H InufusaM Yasutomi
Feb 3, 2000·Cancer Letters·W P MielickiK Chojnowski
Citations
May 15, 2007·Blood·Ajit Varki
Mar 16, 2011·Cardiology Research and Practice·Andrew D Blann, Simon Dunmore
Feb 13, 2007·Critical Reviews in Oncology/hematology·Jeffrey I ZwickerBruce Furie
Nov 9, 2010·Dermatologic Therapy·Gionata BuggianiTorello Lotti
Oct 14, 2018·Cancers·Norbaini Binti Abdol RazakPat Metharom
Jun 23, 2020·Case Reports in Obstetrics and Gynecology·Hiroharu KobayashiHiroshi Adachi
Mar 21, 2021·Thrombosis and Haemostasis·Jun WanMark Roest