Properties of purine nucleoside phosphorylase (PNP) of mammalian and bacterial origin

Zeitschrift Für Naturforschung. C, a Journal of Biosciences
A BzowskaD Shugar

Abstract

Purine nucleoside phosphorylase (PNP), from calf spleen, human erythrocytes and E. coli have been examined with regard to structural requirements of substrates and inhibitors. Kinetic parameters (Km, Vmax/Km) for a variety of N(1) and/or N(7)-methylated analogues of guanosine, inosine and adenosine have been evaluated for all three enzymes. The substrate and/or inhibitor properties of purine riboside, 1,6-dihydropurine riboside, some deazapurine nucleosides: 3-deaza- and 7-deazainosine, 1,3-dideazapurine riboside (ribobenzimidazole), and a variety of acyclonucleosides, have been determined with mammalian and bacterial enzymes. Overall results indicate distinct similarities of kinetic properties and structural requirements of the two mammalian enzymes, although there are some differences as well. The N(1) and O6 of the purine ring are necessary for substrate-inhibitor activity and constitute a binding site for the mammalian (but not the bacterial) enzymes. Moreover, nucleosides lacking the N(3) undergo phosphorolysis and those lacking N(7) are inhibitors (but not substrates). Methylation of the ring N(7) leads to two overlapping effects: labilization of the glycosidic bond, and impediment to protonation at this site by the enzym...Continue Reading

Citations

Nov 15, 2001·The Journal of Biological Chemistry·Gregory A KicskaVern L Schramm
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·You-Na KangSteven E Ealick
Aug 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Javier Suarez, Vern L Schramm
Apr 9, 2020·Biomolecules·Irina A Il'ichevaSergey N Mikhailov
Jun 6, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Vladimir I TimofeevInna P Kuranova
Aug 26, 2003·The Journal of Biological Chemistry·Eric M BennettSteven E Ealick
Jan 15, 1997·European Journal of Biochemistry·B Wielgus-KutrowskaD Shugar
Mar 1, 1997·Analytical Biochemistry·C E RiegerJ L Turnbull
May 5, 2001·Pharmacology & Therapeutics·A BzowskaD Shugar
Jun 15, 1991·Biochemical Pharmacology·A BzowskaN G Johansson
Apr 1, 1992·Journal of Biotechnology·J R Hanrahan, D W Hutchinson
Oct 12, 2004·Biochemical and Biophysical Research Communications·Diego O NolascoDiógenes S Santos
Jul 9, 2021·Journal of Chemical Information and Modeling·Aurélien F A MoumbockStefan Günther
Jun 24, 2010·Bioorganic & Medicinal Chemistry·Rodrigo G DucatiWalter F de Azevedo
May 7, 2009·Archives of Biochemistry and Biophysics·Rodrigo G DucatiLuiz A Basso

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