PMID: 2495250Apr 15, 1989Paper

Properties of recombinant interleukin 2-cultured tumor-infiltrating lymphocytes in human lung cancer

International Journal of Cancer. Journal International Du Cancer
T YanoK Nomoto

Abstract

It is thought that TIL can be activated in vitro by rIL-2 and acquire specific anti-tumor activity. In this study, we investigated this possibility, using lymphocytes isolated from primary lung cancer tissues. In a first series of experiments, TILs and autologous PBLs from 16 patients were cultured in rIL-2 from 7 to 14 days under identical conditions, and were compared for proliferation (16 cases), cytolytic activity (11 cases), gamma interferon (IFN-gamma) production (8 cases), and phenotypes (10 cases). TILs grew in response to rIL-2 as well as PBLs. However, the induced cytolytic activity of TIL was significantly lower than that of PBL against autologous tumor cells and 2 human tumor cell lines. IL-2-mediated IFN-gamma production by TILs was also significantly lower than that of PBLs. TILs were phenotypically characterized by their high CD4/CD8 ratio and lack of Leu11-positive cells. Further investigations with 7 other cases showed that exogenous addition of IFN-gamma to rIL-2 cultures of TILs enhanced cytolytic activity in 4 cases. Our results indicate that IL-2 alone is sufficient for TILs to proliferate but not to acquire new functions (cytotoxicity and production of IFN-gamma).

References

Nov 15, 1979·International Journal of Cancer. Journal International Du Cancer·B M Vose, M Moore
Jul 31, 1985·Biochemical and Biophysical Research Communications·K KatoA Kakinuma
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T W ChangJ Le
Oct 1, 1984·The Journal of Experimental Medicine·G TrinchieriB Perussia

❮ Previous
Next ❯

Citations

Sep 1, 1992·Bulletin of Mathematical Biology·R LefeverP Meyers
Jan 1, 1990·Lung·G SemenzatoV Dipietro
Feb 1, 2002·Surgery·Tokujiro YanoKeizo Sugimachi
Mar 28, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Min XuWei Wang
May 1, 1993·American Journal of Respiratory Cell and Molecular Biology·S G SwisherM D Roth
Mar 12, 1991·International Journal of Cancer. Journal International Du Cancer·I YoshinoK Nomoto
Apr 1, 1993·Mayo Clinic Proceedings·R J Pisani
Jun 6, 2008·International Journal of Cancer. Journal International Du Cancer·Rita RotondoGuido Frumento
Jan 1, 1994·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·G MelioliL Moretta
Jun 23, 2010·Otolaryngologia polska. The Polish otolaryngology·Katarzyna StarskaEwa Głowacka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.