Properties of ShB A-type potassium channels expressed in Shaker mutant Drosophila by germline transformation

Neuron
W N ZagottaR W Aldrich

Abstract

We have used P element-mediated germline transformation to express ShB channels in Shaker mutant Drosophila and have examined their properties by patch-clamp of embryonic myotubes. The transformed ShB cDNA was placed under the transcriptional control of a heat shock promoter (hsp70). Northern blots revealed that transformed DNA is efficiently transcribed in response to heat shock. Cultured myotubes from the transformants produced large A-type potassium currents in response to heat shock. Although qualitatively similar to native Shaker A-currents in wild-type myotubes, transformant A-current inactivates more rapidly and recovers from inactivation more rapidly, similar to ShB channels expressed in Xenopus oocytes. Unlike the channels in oocytes, however, the transformant A-current is insensitive to 50 nM charybdotoxin.

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Citations

Oct 1, 1990·Trends in Neurosciences·L Y Jan, Y N Jan
Nov 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M StockerO Pongs
Sep 1, 1996·The Journal of General Physiology·J J RosenthalW F Gilly
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Jun 18, 2015·Biophysical Journal·Sara de LorenzoFelix Ritort

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