PMID: 2489676Jan 1, 1989Paper

Properties of sialidases from salivary glands, brain, liver and kidney of the rat

Meikai Daigaku shigaku zasshi = The Journal of Meikai University School of Dentistry
A Sato

Abstract

Sialidase (SD, neuraminidase, EC 3.2.1 18) is an enzyme which releases terminal sialic acid residues from glycoproteins, glycolipids nad oligosaccharides. In this study, we report some characteristics of this enzyme in rat salivary glands, brain, liver and kidney. SD activity was measured fluorometrically by using the synthetic substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid (4MU-AcNeu). 1. SD activity in tissue homogenates was maximal at pH 4.0 in the submandibular gland (SMG), and at pH 4.5 in both the sublingual gland (SLG) and parotid gland (PG), the values being very similar to those for the activity in brain, liver and kidney. Enzyme activity was highest in SMG, followed by PG and SLG. However, SD activity was lower in these glands than in brain, liver and kidney. 2. Cell fractionation showed that the intracellular distribution of SD in the salivary glands was mainly in the lysosomal fraction, similar to its distribution in brain, liver or kidney. 3. The pH optimum of lysosomal SD was 4.0 in the SMG, and 4.5 in both SLG and PG. The Km value for 4MU-AcNeu of lysosomal SD from all salivary glands was about 0.09 mM. On the other hand, soluble SD in SMG and PG extracts had its pH optimum at 5.5, and a Km value ...Continue Reading

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