Properties of single calcium-activated potassium channels of large conductance in rat hippocampal neurons in culture

The European Journal of Neuroscience
K T Wann, C D Richards

Abstract

Patch-clamp recordings were made on rat hippocampal neurons maintained in culture. In cell-attached and excised inside-out and outside-out patches a large single-channel current was observed. This channel had a conductance of 220 and 100 pS in 140 mM [K+]i/140 mM [K+]o and 140 mM [K+]i/3 mM [K+]o respectively. From the reversal potential the channel was highly selective for K+, the PK+/PNa+ ratio being 50/1. Channel activity was voltage-dependent, the open probability at 100 nM [Ca2+]i increasing by e-fold for a 22 mV depolarization. It was also dependent on [Ca2+]i at both resting and depolarized membrane potentials. Channel open states were best described by the sum of two exponentials with time constants that increased as the membrane potential became more positive. Channel activity was sensitive to both external (500 microM) and internal (5 mM) tetraethylammonium chloride. These data are consistent with the properties of maxi-K+ channels described in other preparations, and further suggest a role for maxi-channel activity in regulating neuronal excitability at the resting membrane potential. Channel activity was not altered by 8-chlorophenyl thio cAMP, concanavalin A, pH reduction or neuraminidase. In two of five patches le...Continue Reading

References

May 1, 1992·Pflügers Archiv : European journal of physiology·A CarlJ R Hume
Aug 1, 1991·The American Journal of Physiology·A CarlK M Sanders
Aug 1, 1991·The Journal of Physiology·M P Mahaut-Smith, M J Mason
Oct 1, 1991·Current Opinion in Neurobiology·B A Barres
Jan 1, 1991·The Japanese Journal of Physiology·A YoshidaY Ikemoto
Feb 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H Rehm, B L Tempel
Feb 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A PrzywaraA R Wakade
Jun 1, 1986·Journal of Neurophysiology·B Lancaster, P R Adams
Jan 1, 1987·The Journal of Physiology·B Robertson, K T Wann
Jan 1, 1988·Annual Review of Neuroscience·R L Barchi
Dec 1, 1987·Pflügers Archiv : European journal of physiology·D G Lang, A K Ritchie
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P PennefatherR A Nicoll
Jan 1, 1989·Neuron·P H ReinhartI B Levitan
Jan 1, 1989·Annual Review of Physiology·R LatorreO Alvarez
Apr 1, 1986·Journal of Neurophysiology·M Segal, J L Barker
Nov 1, 1986·Biophysical Journal·R S BrettB Lancaster
Jan 22, 1971·Biochemical and Biophysical Research Communications·C L Moore
Jun 1, 1972·Comparative Biochemistry and Physiology. A, Comparative Physiology·R W Meech
Dec 1, 1984·The Journal of Physiology·A MartyA Trautmann
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Oct 1, 1982·The Journal of Physiology·J N BarrettB S Pallotta
Dec 1, 1982·Pflügers Archiv : European journal of physiology·F SachsN Barkakati
Feb 23, 1984·Nature·O H Petersen, Y Maruyama
Dec 1, 1958·Biochimica Et Biophysica Acta·G GARDOS

❮ Previous
Next ❯

Citations

Mar 1, 1996·Pflügers Archiv : European journal of physiology·J M Mienville, J L Barker
Dec 9, 1998·Journal of Pharmacological and Toxicological Methods·P A GoodwinK T Wann
Sep 25, 2009·American Journal of Physiology. Cell Physiology·Neil C HenneyBronwen A J Evans
Oct 24, 2009·Epilepsy Research·Simon Waldbaum, Manisha Patel
Oct 12, 2010·Seminars in Cell & Developmental Biology·Uwe SchulteBernd Fakler
Nov 13, 2014·Applied Immunohistochemistry & Molecular Morphology : AIMM·Pengjuan XuZhuo Yang
Jan 13, 2006·The Journal of Biological Chemistry·Xiao-Fang LiJonathan Lytton
Jun 2, 2006·American Journal of Physiology. Cell Physiology·Brian M Hagen, Kenton M Sanders
Feb 28, 2003·Molecular Pharmacology·Gábor Czirják, Péter Enyedi
Jul 21, 2007·The Journal of Pharmacology and Experimental Therapeutics·Olga OstrovskayaSean M Wilson
Feb 13, 2001·Journal of Neurophysiology·C B Ransom, H Sontheimer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.