PMID: 5179May 14, 1976Paper

Properties of soluble and particulate cysteine sulfinate decarboxylase of the adult and the developing rat brain

Brain Research
H Pasantes-MoralesP Mandel

Abstract

Some properties of cysteine sulfinate decarboxylase (CSD) activity were studied in the pellet and supernatant of a 18,000 X g centrifugation of isotonic sucrose rat brain homogenates. About 50% of the enzyme activity was found associated to the particulate fraction and 16% of the activity remained particle bound after hypo-osmotic shock of the 18,000 X g pellet. The activity of the 18,000 X g supernatant showed a lower dependence on exogenous pyridoxal phosphate (PLP) than the activity in the particulate fraction. The CSD activity of these fractions also differed in optimal pH and in apparent kinetic constants. The enzyme associated to particles showed the highest Vmax and the lowest Km. The activity and kinetic characteristics of CSD were studied during brain postnatal development. In the newborn brain only a small amount of the enzyme activity was found associated to the 18,000 X g pellet. CSD in brain homogenates of immature rats was less dependent on free PLP and showed a higher Km and a lower Vmax as compared with the enzyme in the adult brain. Between birth and adulthood the enzyme activity increased more than 10-fold in the particulate fraction and 2-fold in the soluble fraction. It is concluded that the differences obse...Continue Reading

References

May 1, 1975·Journal of Neurochemistry·D K Rassin, G E Gaull
Apr 1, 1968·Physiological Reviews·J G Jacobsen, L H Smith
Jun 4, 1974·Biochemical and Biophysical Research Communications·H Pasantes-MoralesA Gómez-Puyou
Oct 1, 1972·Journal of Neurochemistry·L K Kaczmarek, A N Davison
Mar 15, 1973·Brain Research·H Pasantes-MoralesP Mandel
Jan 1, 1956·Biochimica Et Biophysica Acta·A N DAVISON
Apr 6, 1964·Biochimica Et Biophysica Acta·J G JACOBSENL H SMITH

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Citations

Jul 1, 1977·Life Sciences·J A SturmanG E Gaull
May 1, 1991·Brain Research. Brain Research Reviews·J B Lombardini
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J A Worden, M H Stipanuk
Jan 1, 1986·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·S OhkumaT Mukainaka
Jul 4, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·T Ide
Nov 1, 1976·Journal of Neurochemistry·H Pasantes-MoralesP Mandel
Nov 1, 1979·Journal of Neurochemistry·P N Gray, S L Dana
Jul 1, 1983·Neurochemical Research·A AbeleJ Mark
Jan 1, 1989·Progress in Neurobiology·R J Huxtable
Sep 4, 2015·Neurochemistry International·Ingeborg WingeJan Haavik
Sep 1, 1982·Journal of Neurochemistry·E HamelE Roberts
Jan 1, 1980·Journal of Neuroscience Research·W A StainesE G McGeer
Nov 26, 1999·The Journal of Nutrition·T ParkJ G Morris

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