PMID: 7032910Nov 1, 1981Paper

Properties of sorbitol dehydrogenase and characterization of a reactive cysteine residue reveal unexpected similarities to alcohol dehydrogenases

European Journal of Biochemistry
J JefferyH Jörnvall

Abstract

Sorbitol dehydrogenase was characterized as a homogeneous protein on affinity chromatography and ion-exchange chromatography. Tests of stability, sensitivity to inhibitors, and protection by coenzyme suggest that the enzyme has essential cysteine, metal, and probably histidine. The native enzyme has a molecular weight around 140 000 and a subunit around 35 000--40 000, suggesting a tetrameric quaternary structure. Subunits are highly similar if not identical as judged by characterization of one unique 45-residue sequence containing a single reactive cysteine residue. Properties resemble those of mammalian and yeast alcohol dehydrogenases, and the sequence determined for the region around the reactive cysteine residue is homologous to that around one of the zinc-liganding cysteine residues at the active site of horse liver alcohol dehydrogenase. Sorbitol dehydrogenase thus reveals an unexpected relationship to alcohol dehydrogenases, from which ancestral connections and functional mechanisms in this group of enzymes may be further elucidated.

References

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Citations

May 1, 1997·Protein Science : a Publication of the Protein Society·M PeretzY Burstein
Feb 1, 1991·Journal of Protein Chemistry·T BergmanJ Jeffery
Jan 1, 1983·The International Journal of Biochemistry·J N Burnell, R S Holmes
Jan 1, 1983·The International Journal of Biochemistry·N C Leissing, E T McGuinness
Jan 1, 1983·Pharmacology, Biochemistry, and Behavior·H JörnvallJ Jeffery
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·L J JiangB L Vallee
Apr 2, 1984·European Journal of Biochemistry·J JefferyH Jörnvall
Apr 2, 1984·European Journal of Biochemistry·H JörnvallJ Jeffery
Dec 22, 1989·European Journal of Biochemistry·C KarlssonH Jörnvall
Feb 1, 1993·European Journal of Biochemistry·H ReiersenJ S McKinley-McKee
Oct 1, 1996·European Journal of Biochemistry·R I Lindstad, J S McKinley-McKee
Sep 10, 2003·Structure·Thomas A PaulyVirginia L Rath
Jun 15, 2016·ACS Applied Materials & Interfaces·Lin ZhangMathieu Etienne
Sep 5, 1989·Biochemistry·M C Feiters, J Jeffery
Apr 1, 1993·European Journal of Biochemistry·P RouimiH Jörnvall
Feb 1, 1987·Current Eye Research·J JedziniakU Andley
Apr 15, 1997·Archives of Biochemistry and Biophysics·I MariniU Mura

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