Properties of Streptomyces albus J1074 mutant deficient in tRNALeuUAA gene bldA

Archives of Microbiology
Oksana KoshlaB Ostash

Abstract

Streptomyces albus J1074 is one of the most popular and convenient hosts for heterologous expression of gene clusters directing the biosynthesis of various natural metabolic products, such as antibiotics. This fuels interest in elucidation of genetic mechanisms that may limit secondary metabolism in J1074. Here, we report the generation and initial study of J1074 mutant, deficient in gene bldA for tRNALeuUAA, the only tRNA capable of decoding rare leucyl TTA codon in Streptomyces. The bldA deletion in J1074 resulted in a highly conditional Bld phenotype, with depleted formation of aerial hyphae on certain solid media. In addition, bldA mutant of J1074 was unable to produce endogenous antibacterial compounds and two heterologous antibiotics, moenomycin and aranciamycin, whose biosynthesis is directed by TTA-containing genes. We have employed a new TTA codon-specific β-galactosidase reporter system to provide genetic evidence that J1074 bldA mutant is impaired in translation of TTA. In addition, we have discussed the possible reasons for differences in the phenotypes of bldA mutants described here and in previous studies, providing knowledge to study bldA-based regulation of antibiotic biosynthesis.

References

Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·B K LeskiwK F Chater
Jun 1, 1986·Journal of General Microbiology·A A King, K F Chater
Sep 1, 1987·Journal of General Microbiology·J Majer, K F Chater
Feb 1, 1980·Journal of General Microbiology·K F Chater, L C Wilde
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 23, 2000·Molecular & General Genetics : MGG·A BurgerW Wohlleben
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertolt GustKeith F Chater
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen
Mar 16, 2007·Chembiochem : a European Journal of Chemical Biology·Andriy LuzhetskyyAndreas Bechthold
Jun 14, 2008·Gene·Marta FedoryshynAndriy Luzhetskyy
Sep 30, 2008·Trends in Genetics : TIG·Arnold KuzniarJack A M Leunissen
Jul 4, 2009·Bioinformatics·Nestor ZaburannyyVictor Fedorenko
Mar 6, 2010·Journal of Industrial Microbiology & Biotechnology·Roman MakitrynskyyVictor Fedorenko
May 15, 2010·Journal of Industrial Microbiology & Biotechnology·Richard H Baltz
Jan 20, 2011·Molecular Microbiology·B M Fredrik Pettersson, Leif A Kirsebom
Jan 17, 2012·Applied and Environmental Microbiology·Simone HerrmannAndriy Luzhetskyy
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Gang LiuHuarong Tan
May 30, 2013·Molecular and Cellular Biology·Tek N LamichhaneRichard J Maraia
Oct 30, 2013·Molecular Microbiology·Bohdan OstashSuzanne Walker
Jan 30, 2014·Applied Microbiology and Biotechnology·Maksym MyronovskyiAndriy Luzhetskyy
Feb 6, 2014·BMC Genomics·Nestor ZaburannyiAndriy Luzhetskyy
Feb 26, 2014·Applied Microbiology and Biotechnology·Bohdan Bilyk, Andriy Luzhetskyy
Jul 12, 2014·Applied Microbiology and Biotechnology·Liliya HorbalAndriy Luzhetskyy
Jul 23, 2016·Journal of the American Chemical Society·Hala A IqbalSean F Brady

❮ Previous
Next ❯

Citations

Feb 20, 2018·Microbial Cell Factories·Dimitris KallifidasHendrik Luesch
Mar 20, 2018·Applied Microbiology and Biotechnology·Bingbing HouHuizhan Zhang
Jan 9, 2020·Journal of Applied Genetics·Oleksandr YushchukVictor Fedorenko
Mar 18, 2021·World Journal of Microbiology & Biotechnology·Oksana KoshlaBohdan Ostash

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

REDIRECT

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.