PMID: 3768296Aug 12, 1986Paper

Properties of the alkali light-chain-20-kilodalton fragment complex from skeletal myosin heads

Biochemistry
P ChaussepiedI P Trayer

Abstract

We have developed a rapid and reproducible procedure widely applicable to the preparation of pure aqueous solutions of the complex between an alkali light chain and the COOH-terminal heavy-chain fragments of skeletal myosin chymotryptic subfragment 1 (S-1) split by various proteases. It was founded on the remarkable ethanol solubility of these complexes. A systematic study of the ethanol fractionation of the tryptic (27K-50K-20K)-S-1 (A2) showed the NH2-terminal 27K fragment to behave like a specific protein entity being quantitatively precipitated at a relatively low ethanol concentration. Only the 20K peptide-A2 complex remained in solution when the S-1 derivative was treated with exactly 4 volumes of ethanol in the presence of 6 M guanidinium chloride. At a lower ethanol concentration, a soluble mixture of 50K and 20K peptides together with the light chain was obtained. The isolated 20K fragment-A2 system containing a 1:1 molar ratio of each component was investigated by biochemical and 1H nuclear magnetic resonance (NMR) techniques to highlight its structure and the interaction of the 20K heavy-chain segment with F-actin and with the light chain. During the treatment of the complex with alpha-chymotrypsin, only the 20K pept...Continue Reading

References

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Citations

Aug 20, 1987·Journal of Molecular Biology·L CastellaniC Cohen
Nov 3, 2001·Biochimica Et Biophysica Acta·D Mornet, A Bonet-Kerrache
Jun 1, 1992·Journal of Muscle Research and Cell Motility·B CornillonJ Leger
Apr 15, 1988·Biochemical and Biophysical Research Communications·A BonetD Mornet
Feb 20, 1990·Biochemistry·A Bonet-KerracheD Mornet
Jun 1, 1990·Journal of Muscle Research and Cell Motility·M Dan-GoorA Muhlrad
Jun 1, 1988·Journal of Muscle Research and Cell Motility·E AudemardD Mornet
Feb 1, 1993·Journal of Muscle Research and Cell Motility·S S MargossianH S Slayter
Aug 1, 1988·Journal of Muscle Research and Cell Motility·P Vibert, C Cohen

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