PMID: 108242Mar 1, 1979

Properties of the Bacillus subtilis spore coat

Journal of Bacteriology
N K Pandey, A I Aronson


About 70% of the protein in isolated Bacillus subtilis spore coats was solubilized by treatment with a combination of reducing and denaturing agents at alkaline pH. The residue, consisting primarily of protein, was insoluble in a variety of reagents. The soluble proteins were resolved into at least seven bands by sodium dodecyl sulfate gel electrophoresis. About one-half of the total was four proteins of 8,000 to 12,000 daltons. These were relatively tyrosine rich, and one was a glycoprotein. There was also a cluster of proteins of about 40,000 daltons and two or three in the 20,000- to 25,000-dalton range. The insoluble fraction had an amino acid composition and N-terminal pattern of amino acids very similar to those of the soluble coat proteins. A major difference was the presence of considerable dityrosine in performic acid-oxidized preparations of insoluble coats. Coat antigen including a 60,000-dalton protein not present in extracts of mature spores was detected in extracts of sporulating cells by immunoprecipitation. This large antigen turned over in a pulse-chase experiment. Antibodies to either the array of 8,000- to 12,000-dalton coat polypeptides or to the larger coat proteins reacted with this 60,000-dalton species, ...Continue Reading


Dec 27, 2002·The Journal of General and Applied Microbiology·Katsunori KobayashiShigeru Yamanaka
Oct 18, 2008·Applied Spectroscopy·Rohit BhartiaPamela G Conrad
Dec 19, 2013·Archives of Microbiology·Elena VenirMara Lucia Stecchini
Feb 14, 2015·Biotechnology and Bioengineering·Xia WuJonathan S Dordick
May 1, 1981·Archives of Microbiology·O P Srivastava, A I Aronson
Sep 3, 2004·Journal of Bacteriology·Lyethur S ErlendssonLars Hederstedt
Mar 1, 1992·Journal of Fluorescence·A J KunglH F Kauffmann
Feb 7, 2020·International Journal of Molecular Sciences·Grzegorz JanuszAndrzej Paszczyński
Jan 1, 1988·Microbiology and Immunology·M ImagawaM Kondo
Aug 1, 1982·The Journal of Applied Bacteriology·A H Dadd, G M Daley
Aug 20, 2004·Journal of Bacteriology·Katerina Ragkousi, P Setlow
Aug 19, 2003·Journal of Bacteriology·Samuel AmiteyeTsutomu Sato
Jul 1, 1996·European Journal of Biochemistry·P BrizaM Breitenbach
Nov 4, 2005·Journal of Bacteriology·Rita ZilhãoAdriano O Henriques
Nov 24, 2007·Annual Review of Microbiology·Adriano O Henriques, Charles P Moran
Sep 8, 2010·Applied and Environmental Microbiology·Rohit BhartiaKenneth H Nealson
Jan 1, 1987·Microbiology and Immunology·H I el-BelbasiM Kondo
Nov 10, 2013·Biotechnology and Bioengineering·Ruchir V MundraJonathan S Dordick
May 12, 2012·Journal of Applied Microbiology·M J LeggettJ-Y Maillard
Jan 5, 2006·Molecular Microbiology·Hosan KimAdam Driks
Nov 1, 2016·Comprehensive Reviews in Food Science and Food Safety·Aswathi SoniPhil Bremer

Related Concepts

Antigens, Bacterial
Natto Bacteria
Bacterial Proteins
Cell Wall
Spores, Bacterial

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.