Properties of the cytoplasmic progestin-binding protein in the rabbit uterus

Endocrinology
D PhilibertJ P Raynaud

Abstract

An exchange assay for the measurement of total cytoplasmic progestin binding sites has been developed on rabbit uterine cytosol using the highly potent progestin, R5020 (17,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione) labelled to a high specific activity. This compound has several advantages over progesterone: it is not bound by plasma corticosteroid binding globulin; it has high affinity for the progestin receptor; it binds virtually as fast as progesterone to the receptor, but the complex formed dissociated 8 times slower; its binding is not displaced by more than 2% by compounds devoid of progestational activity (estrogens, testosterone, dexamethasone, aldosterone). Bound endogenous progesterone was exchanged by tritiated R 5020 in a time compatible with receptor stability. At 0 C, total exchange of filled sites occurred in less than 4 h; at this temperature the R 5020-receptor complex was stable for at least 28 h. The conformation of the R 5020-receptor complex was investigated in sucrose density gradients under various experimental conditions. Unlike progesterone, it was possible to detect a 7S peak in uterine cytosol obtained from rabbits injected with a tracer dose of [3H]R 5020 1 h prior to sacrifice.

Citations

Jan 1, 1984·Histochemistry·R BochskanlC Kirchner
Mar 23, 1979·Brain Research·M Moguilewsky, J P Raynaud
Aug 1, 1979·Journal of Steroid Biochemistry·H G VerhageR C Jaffe
Jun 1, 1980·Journal of Steroid Biochemistry·F Luzzani, A Soffientini
Aug 1, 1983·Journal of Steroid Biochemistry·D J Lamb, D W Bullock
Jan 1, 1986·Journal of Steroid Biochemistry·L A van der Walt, J L Wittliff
Jan 1, 1988·Journal of Steroid Biochemistry·N TeradaT Oka
Oct 1, 1978·Molecular and Cellular Endocrinology·M Warembourg
Dec 1, 1980·Molecular and Cellular Endocrinology·C E YoungD W Bullock
Apr 1, 1981·Molecular and Cellular Endocrinology·C E YoungD W Bullock
May 1, 1983·Molecular and Cellular Endocrinology·D H Seeley, P D Costas
Jul 1, 1986·Molecular and Cellular Endocrinology·S M Ho, V Levin
Feb 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·E Di SalleA Panzeri
Apr 1, 1985·Journal of Endocrinological Investigation·H J GrillK Pollow
Jun 1, 1987·American Journal of Obstetrics and Gynecology·L E FaberJ L Duhring
Mar 1, 1986·General and Comparative Endocrinology·S M Kleis-San Francisco, I P Callard

Related Concepts

Cytoplasmic Matrix
Drug Stability
Estradiol, (16 alpha,17 beta)-Isomer
Hydrocortisone, (9 beta,10 alpha,11 alpha)-Isomer
Progesterone, (9 beta,10 alpha)-Isomer
Receptors, Progesterone
Uterus

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.