PMID: 8955314Dec 1, 1996Paper

Properties of the FhuA channel in the Escherichia coli outer membrane after deletion of FhuA portions within and outside the predicted gating loop

Journal of Bacteriology
H KillmannV Braun

Abstract

Escherichia coli transports Fe3+ as a ferrichrome complex through the outer membrane in an energy-dependent process mediated by the FhuA protein. A FhuA deletion derivative lacking residues 322 to 355 (FhuA delta322-355) forms a permanently open channel through which ferrichrome diffused. This finding led to the concept that the FhuA protein forms a closed channel that is opened by input of energy derived from the electrochemical potential across the cytoplasmic membrane, mediated by the Ton system. In this study, we constructed various FhuA derivatives containing deletions inside and outside the gating loop. FhuA delta322-336 bound ferrichrome and displayed a residual Ton-dependent ferrichrome transport activity. FhuA delta335-355 no longer bound ferrichrome but supported ferrichrome diffusion through the outer membrane in the absence of the Ton system. FhuA delta335-355 rendered cells sensitive to sodium dodecyl sulfate and supported diffusion of maltotetraose and maltopentaose in a lamB mutant lacking the maltodextrin-specific channel in the outer membrane. Cells expressing FhuA delta70-223, which has a large deletion outside the gating loop, were highly sensitive to sodium dodecyl sulfate and grew on maltodextrins but showe...Continue Reading

References

Jul 1, 1978·Journal of Bacteriology·K Hantke, V Braun
Dec 1, 1991·Molecular Microbiology·J T Skare, K Postle
Dec 1, 1990·Molecular Microbiology·K Postle
Mar 1, 1986·Journal of Bacteriology·R BenzG H Vos-Scheperkeuter
May 1, 1985·Journal of Bacteriology·R BenzR E Hancock
Jan 1, 1988·Annual Review of Microbiology·R Benz
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Jan 1, 1984·Molecular & General Genetics : MGG·T OlschlägerV Braun
Feb 1, 1980·Archives of Microbiology·H J Krieger-Brauer, V Braun
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J LiuP E Klebba
Jan 1, 1993·Molecular Microbiology·M StruyvéJ Tommassen
Apr 1, 1993·Molecular Microbiology·M KarlssonC F Higgins

❮ Previous
Next ❯

Citations

Jun 27, 1998·Molecular Microbiology·G S Moeck, J W Coulton
Oct 18, 2002·European Journal of Biochemistry·Michael BraunVolkmar Braun
Aug 1, 1997·Biological Chemistry·T E Creighton
Mar 21, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Volkmar Braun, Franziska Endriss
Jul 19, 2012·Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology·Antonio Carvajal-Rodríguez
Jul 27, 2011·Biochemical Society Transactions·Benjamin C KrasityMargaret J McFall-Ngai
Dec 30, 2010·The Journal of Biological Chemistry·Mohammad M MohammadLiviu Movileanu
Feb 24, 2004·Infection and Immunity·Mary Kate Yost-Daljev, Cynthia Nau Cornelissen
Mar 5, 2004·Journal of Bacteriology·Anne Marie AugustusRajeev Misra
Mar 19, 2008·Infection and Immunity·Jennifer M Noto, Cynthia Nau Cornelissen
May 10, 2001·Journal of Bacteriology·H KillmannV Braun
Mar 23, 2006·Infection and Immunity·James M FleckensteinMichael Burkitt
Sep 8, 1998·Current Opinion in Structural Biology·R M StroudD Freymann
Aug 17, 2017·Biochemistry·Avinash Kumar ThakurLiviu Movileanu
May 30, 1998·Journal of Medicinal Chemistry·R NudelmanA Shanzer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.