Properties of the glucose-6-phosphate transporter from Chlamydia pneumoniae (HPTcp) and the glucose-6-phosphate sensor from Escherichia coli (UhpC).

Journal of Bacteriology
Christian SchwöppeH Ekkehard Neuhaus

Abstract

The amino acid sequence of the proposed glucose-6-phosphate (Glc6P) transporter from Chlamydia pneumoniae (HPTcp; hexose phosphate transporter [Chlamydia pneumoniae]) exhibits a higher degree of similarity to the Escherichia coli Glc6P sensor (UhpC) than to the E. coli Glc6P transporter (UhpT). Overexpression of His-UhpC in a UhpT-deficient E. coli strain revealed that the sensor protein is also able to transport Glc6P and exhibits an apparent K(m) ((Glc6P)) of 25 microM, whereas His-HPTcp exhibits an apparent K(m)( (Glc6P)) of 98 microM. His-HPTcp showed a four-times-lower specific activity than His-UhpT but a 56-times-higher specific activity than His-UhpC. Like His-UhpT and His-UhpC, the carrier His-HPTcp performs a sugar-phosphate/inorganic-phosphate antiporter mode of transport. Surprisingly, while physiological concentrations of inorganic phosphate competitively inhibited transport mediated by the E. coli proteins His-UhpT and His-UhpC, transport mediated by His-HPTcp was not inhibited. Interestingly, C(3)-organophosphates stimulated His-HPTcp activity but not His-UhpT- or His-UhpC-catalyzed Glc6P transport. In contrast to His-UhpC, the His-HPTcp protein does not act as a Glc6P sensor in the uhp regulon.

References

Jan 1, 1976·Advances in Enzymology and Related Areas of Molecular Biology·G W Dietz
Mar 1, 1991·Microbiological Reviews·J W Moulder
May 1, 1982·Journal of Bacteriology·T P HatchJ A Silverman
Apr 1, 1984·Journal of Bacteriology·P C MaloneyL Schiller
Jan 1, 1993·Trends in Biochemical Sciences·M D Marger, M H Saier
Dec 5, 1998·The Journal of Biological Chemistry·M C Fann, P C Maloney
Oct 13, 2000·Molecular Microbiology·E R Iliffe-Lee, G McClarty
Apr 28, 2001·Journal of Bacteriology·J S Wright, R J Kadner

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Citations

Mar 24, 2004·Current Opinion in Microbiology·Agathe Subtil, Alice Dautry-Varsat
Apr 9, 2005·Trends in Microbiology·Klaas J Hellingwerf
Oct 16, 2013·Trends in Plant Science·Fabio FacchinelliAndreas P M Weber
Dec 9, 2008·Nature Chemical Biology·Griet Van ZeebroeckJohan M Thevelein
Mar 26, 2003·European Journal of Biochemistry·Christian SchwöppeH Ekkehard Neuhaus
Nov 13, 2008·The Journal of Biological Chemistry·Oliver TrentmannIlka Haferkamp
Sep 21, 2004·Annual Review of Plant Biology·Sylvie LalondeWolf B Frommer
Sep 1, 2007·PLoS Biology·Oliver TrentmannIlka Haferkamp
Apr 9, 2008·Proceedings of the National Academy of Sciences of the United States of America·Yuichi HongohMoriya Ohkuma
Jan 1, 2002·The Arabidopsis Book·Uwe Ludewig, Wolf B Frommer
Nov 12, 2013·Trends in Plant Science·Agathe SubtilMatthias Horn
Feb 6, 2016·Journal of Medicinal Chemistry·James A D GoodFredrik Almqvist
Dec 17, 2008·Analytical Biochemistry·Hilal Taymaz-NikerelWalter M van Gulik
Jun 6, 2003·Molecular Microbiology·Milton H Saier
Aug 28, 2009·Molecular Microbiology·Larissa Tetsch, Kirsten Jung
May 30, 2012·Plant Biology·I Haferkamp, N Linka
Mar 17, 2016·ELife·Lena GehreAgathe Subtil
Feb 12, 2014·Current Opinion in Microbiology·Ake Västermark, Milton H Saier
Dec 21, 2016·Molecular Microbiology·Adrian MehlitzThomas Rudel
Mar 1, 2018·Annual Review of Plant Biology·Eva C M Nowack, Andreas P M Weber
Mar 7, 2007·Science Progress·Martijn BekkerKlaas J Hellingwerf
May 23, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fuping SongChristina Nielsen-LeRoux
Jul 29, 2017·Frontiers in Cellular and Infection Microbiology·Wolfgang EisenreichWerner Goebel
Oct 28, 2019·Frontiers in Microbiology·Arlieke GitselsDaisy Vanrompay
Sep 10, 2020·Infection and Immunity·Rachel J Ende, Isabelle Derré

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