Apr 8, 1976

Properties of the major carboxypeptidase in the larvae of the webbing clothes moth, Tineola bisselliella

Biochimica Et Biophysica Acta
C W Ward

Abstract

The larvae of the webbing clothes moth, Tineola bisselliella contain two carboxypeptidases (EC 3.4.12-) and one of these has been purified by preparative polyacrylamide gel electrophoresis. Its pH optimum for the hydrolysis of N-benzyloxycarbonyl-glycyl-leucine was pH 7.5-7.7 and its molecular weight as judged by gel filtration was 72 000. It is strongly inhibited by disopropylfluorophosphate, thiol reagents and some metal cations and also by 1:10 phenanthroline but not EDTA. Km and V values for the hydrolysis of 13 N-acyl dipeptides were determined. The enzyme has a strong preference for neutral aliphatic amino acid residues and does not hydrolyse C-terminal proline, arginine or lysine. It is a true carboxypeptidase, requiring an L-amino acid in the C-terminal position, with a free carboxyl group and hydrolysing peptide substrates consecutively from the C-terminal end. Dipeptides are cleaved much more slosly than tripeptides or N-acyl dipeptides.

Mentioned in this Paper

Cations, Divalent
Diuron
Structure-Activity Relationship
Antheraea
Iodoacetic Acids
Carboxypeptidase
Tadpole
Chloromercuribenzoates
Hydrogen-Ion Concentration
Edetic Acid, Calcium, Sodium Salt

About this Paper

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