PMID: 7516463Oct 1, 1993Paper

Properties of the ryanodine receptor present in the sarcoplasmic reticulum from lobster skeletal muscle

Membrane Biochemistry
E OlivaresE Rojas

Abstract

Microsomal sarcoplasmic reticulum (SR) fractions from lobster skeletal muscle were found to bind [3H]-ryanodine. [3H]-ryanodine binding was enhanced by AMP, Ca2+ and caffeine, and significantly diminished by ATP, Ba2+ and Sr2+. Furthermore, dantrolene and ruthenium red, two classical inhibitors of Ca2+ release from the SR, blocked [3H]-ryanodine binding. Similarly, tetracaine, known to block the charge movement associated with excitation-contraction coupling in vertebrate muscle, inhibited the binding of the alkaloid. Our lobster SR preparation exhibited a single high-affinity ryanodine binding site (Kd = 6.6 nM, Bmax = 10 pmol/mg protein). Since SDS-PAGE of the SR proteins revealed a major band c. 565 kDa which comigrated with the putative ryanodine receptor from both rat and chicken skeletal muscle, we concluded that lobster skeletal muscle is equipped with the 565 kDa ryanodine receptor. Finally, incorporation of the SR microsomal fraction from lobster into planar bilayer membranes revealed the presence of a ryanodine-sensitive Ca2+ channel activity (160 pS in symmetrical 200 mM CsCl solutions). We concluded that both the crustacean and vertebrate skeletal muscle ryanodine receptor share the relevant properties such as molec...Continue Reading

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Citations

Nov 1, 1996·American Journal of Physical Anthropology·P B SamollowJ Rogers
Sep 1, 1966·Progress in Cardiovascular Diseases·G A Langer
Mar 23, 2000·Insect Biochemistry and Molecular Biology·E PuenteJ D Windass
Feb 2, 2010·Nature Structural & Molecular Biology·Yukiko MuroiBaron Chanda
Apr 29, 1993·The New England Journal of Medicine·A M Katz
Feb 16, 2005·Astrobiology·Andrew PohorilleMichael A Wilson
Feb 24, 2004·Annual Review of Physiology·James S Trimmer, Kenneth J Rhodes
Feb 16, 2005·Annual Review of Physiology·Xander H T WehrensAndrew R Marks
Oct 29, 2013·Cellular and Molecular Life Sciences : CMLS·Julie A Mustard
Mar 5, 1998·The American Journal of Physiology·K E QuinnB E Ehrlich

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