PMID: 2110655Apr 11, 1990Paper

Properties of the transcriptional enhancer in Saccharomyces cerevisiae telomeres

Nucleic Acids Research
K W Runge, V A Zakian

Abstract

Saccharomyces cerevisiae chromosomes end with the sequence C2-3A(CA)1-4, commonly abbreviated as C1-3A. These sequences can function as upstream activators of transcription (UAS's) when placed in front of a CYC1-lacZ fusion gene. When C1-3A sequences are placed between the GAL1,10 UAS and the CYC1-lacZ fusion, the C1-3A UAS still functions and the amount of beta-galactosidase produced in cells grown on glucose is as much or more than that for cells grown on either glycerol medium, or cells grown on glucose medium containing a plasmid with just the C1-3A UAS. These data indicate that the UAS is immune from glucose repression from the upstream GAL1,10 UAS. Because C1-3A sequences are bound in vitro by the transcription factor RAP1, the UAS activity of yeast telomere sequences was compared with that of a similar UAS from the tightly regulated ribosomal protein gene RP39A, which also contains a RAP1 binding site. While transcription from the ribosomal protein gene UAS was responsive to cell density, the amount of transcription from the C1-3A UAS was nearly the same at all cell densities tested. These data show that the transcriptional activation by C1-3A sequences is not regulated by cell density.

References

Jan 1, 1985·Current Genetics·R J LeerR J Planta

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Citations

May 11, 1995·Nucleic Acids Research·J H Wright, V A Zakian
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·M A WhiteT D Petes
Jun 28, 2000·Nucleic Acids Research·C E KoeringE Gilson
Jun 1, 1991·Molecular and Cellular Biology·K W RungeV A Zakian
Feb 1, 1992·Genes & Development·J H WrightV A Zakian

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