Properties of two multifunctional plant fatty acid acetylenase/desaturase enzymes

European Journal of Biochemistry
Anders S CarlssonSten Stymne

Abstract

The properties of the Delta6 desaturase/acetylenase from the moss Ceratodon purpureus and the Delta12 acetylenase from the dicot Crepis alpina were studied by expressing the encoding genes in Arabidopsis thaliana and Saccharomyces cerevisiae. The acetylenase from C. alpinaDelta12 desaturated both oleate and linoleate with about equal efficiency. The desaturation of oleate gave rise to 9(Z),12(E)- and 9(Z),12(Z)-octadecadienoates in a ratio of approximately 3 : 1. Experiments using stereospecifically deuterated oleates showed that the pro-R hydrogen atoms were removed from C-12 and C-13 in the introduction of the 12(Z) double bond, whereas the pro-R and pro-S hydrogen atoms were removed from these carbons during the formation of the 12(E) double bond. The results suggested that the Delta12 acetylenase could accommodate oleate having either a cisoid or transoid conformation of the C(12)-C(13) single bond, and that these conformers served as precursors of the 12(Z) and 12(E) double bonds, respectively. However, only the 9(Z),12(Z)-octadecadienoate isomer could be further desaturated to 9(Z)-octadecen-12-ynoate (crepenynate) by the enzyme. The evolutionarily closely related Delta12 epoxygenase from Crepis palaestina had only weak d...Continue Reading

References

Aug 1, 1970·The Biochemical Journal·L J Morris
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·P BrounC Somerville
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·E B CahoonA J Kinney
Jun 6, 2003·The Plant Journal : for Cell and Molecular Biology·Edgar B CahoonRobert E Minto
Aug 28, 2003·Journal of the American Chemical Society·Darwin W ReedPatrick S Covello
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jan 14, 2004·The Journal of Biological Chemistry·Edgar B Cahoon, Anthony J Kinney

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Citations

Sep 28, 2007·Natural Product Reports·Peter H Buist
Oct 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Montserrat SerraGemma Fabriàs
Apr 5, 2008·Progress in Lipid Research·Robert E Minto, Brenda J Blacklock
Oct 10, 2015·Journal of Agricultural and Food Chemistry·Corinna DawidThomas Hofmann
Feb 13, 2018·Plant Reproduction·Sébastien Baud
Mar 26, 2010·Natural Product Reports·Zhao-Xun Liang
Sep 26, 2006·The Journal of Organic Chemistry·José-Luis AbadGemma Fabriàs

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