Property modelling of lysozyme-crosslinker-alginate complexes using latent variable methods.

Journal of Biomedical Materials Research. Part a
Vida RahmaniHeather Sheardown

Abstract

Statistical methods were used to provide insight into a polymer complex system composed of lysozyme and alginate to quantify the effects of such parameters as pH, and ionic composition of the mixing solution on the properties of the complexes including composition, particle diameter, and zeta potential. Various crosslinkers (calcium, barium, iron[III], and bovine serum albumin), were used with lysozyme for complex formation to investigate the effect of crosslinker charge density on protein release kinetics, modelled using ktn . Multivariate statistical analysis showed that the kinetic parameters associated with the release were, not surprisingly highly dependent on the ionic strength of the release media, with higher ionic strength leading to faster release. The release parameter k was also shown to depend on the protein properties (size, ionic strength) while n was slightly, but not statistically dependent on the charge density of the crosslinker demonstrating that the nature of the crosslinker had minimal impact on drug release. The multivariate statistical has the potential to be used for optimization of the complexes and prediction of physical properties and degradation rates.

References

Aug 10, 2000·Protein Science : a Publication of the Protein Society·C N PaceK L Shaw
Apr 12, 2001·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·P Costa, J M Sousa Lobo
May 4, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Frank GuRonald Neufeld
Dec 13, 2006·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·L A Wells, H Sheardown
Jul 27, 2007·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Maja Simonoska CrcarevskaKaterina Goracinova
Jun 26, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Luciana Lisa LaoNicholas A Peppas
Nov 9, 2010·Advances in Colloid and Interface Science·Christophe Schmitt, Sylvie L Turgeon
Nov 17, 2011·Chemical Society Reviews·Shoshy Mizrahy, Dan Peer
Nov 30, 2011·Progress in Polymer Science·Kuen Yong Lee, David J Mooney
Sep 15, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Jorrit J WaterLene Jorgensen
Feb 19, 2015·Current Protein & Peptide Science·Juan P Fuenzalida, Francisco M Goycoolea
May 14, 2016·Biomacromolecules·Hadas Hecht, Simcha Srebnik
Nov 25, 2017·International Journal of Pharmaceutics·Vida Rahmani, Heather Sheardown

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