Property of thimerosal-induced decrease in cellular content of glutathione in rat thymocytes: a flow cytometric study with 5-chloromethylfluorescein diacetate

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Toshiko Ueha-IshibashiYoshiro Okano

Abstract

There is a concern on the part of public health community that adverse health consequences by thimerosal, a preservative in vaccines for infants, may occur among infants during immunization schedule. Therefore, the effect of thimerosal on cellular content of glutathione was examined on thymocytes obtained from 4-week-old rats using a flow cytometer and 5-chloromethylfluorescein diacetate. Thimerosal at concentrations ranging from 1 to 10 microM reduced the cellular content of glutathione in a concentration-dependent manner, and the complete depletion of cellular glutathione was observed when the cells were treated with 30 microM thimerosal. L-Cysteine significantly attenuated the actions of thimerosal to reduce the glutathione content and to increase the intracellular Ca2+ concentration. Prolonged incubation (24 h) with 1-3 microM thimerosal induced the apoptosis. The cytotoxic action of thimerosal was greatly augmented when the cells suffered oxidative stress induced by H2O2. It may be unlikely that thimerosal exerts potent cytotoxic action under the in vivo condition because the blood concentration of thimerosal after receiving vaccines does not seem to reach micromolar range and nonprotein thiols at micromolar concentrations...Continue Reading

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Citations

Jan 18, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Arbace OfficiosoCaterina Manna
Jun 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Erika HashimotoYasuo Oyama
Jan 15, 2005·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T Ueha-IshibashiY Okano
Nov 26, 2014·The Journal of Toxicological Sciences·Masanori HorieHitoshi Iwahashi
Mar 7, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Dilek Eke, Ayla Celik
Aug 24, 2013·International Journal of Environmental Research and Public Health·Janet K KernMark R Geier
Apr 22, 2005·Toxicological Sciences : an Official Journal of the Society of Toxicology·Damani K ParranMarion Ehrich
Apr 21, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Michelle L HerdmanKinsley Kelley Kiningham

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