Prophage Excision in Streptococcus pneumoniae Serotype 19A ST320 Promote Colonization: Insight Into Its Evolution From the Ancestral Clone Taiwan 19F-14 (ST236)

Frontiers in Microbiology
Yi-Yin ChenYu-Chia Hsieh

Abstract

Streptococcus pneumoniae 19A ST320, a multidrug-resistant strain with high disease severity that notoriously spread before the use of expanded pneumococcal conjugate vaccines, was derived from a capsular switching event between an international strain Taiwan 19F-14 (ST236) and a serotype 19A strain. However, the molecular mechanisms underlying the adaptive evolution of 19F ST236 to 19A ST320 are unknown. In this study, we compared 19A ST320 to its ancestral clone, 19F ST236, in terms of adherence to respiratory epithelial cells, whole transcriptome, and ability to colonize a young mouse model. Serotype 19A ST320 showed five-fold higher adherence to A549 cells than serotype 19F ST236. High-throughput mRNA sequencing identified a prophage region located between dnaN and ychF in both strains; however, the genes in this region were expressed at significantly higher levels in 19A ST320 than in 19F ST236. Analysis by polymerase chain reaction (PCR) showed that the prophage is able to spontaneously excise from the chromosome and form a circular episome in 19A ST320, but not in 19F ST236. Deletion of the integrase in the prophage of 19A ST320 decreased spontaneous excision and cell adherence, which were restored by complementation. Com...Continue Reading

References

Jun 28, 1996·Science·M K Waldor, J J Mekalanos
Sep 25, 1998·Trends in Microbiology·M K Waldor
Jul 27, 2002·Journal of Bacteriology·Forest Rohwer, Rob Edwards
Feb 9, 2008·Emerging Infectious Diseases·Eun Hwa ChoiHoan Jong Lee
Apr 19, 2008·The Journal of Infectious Diseases·Matthew R MooreBernard Beall
Apr 22, 2008·BMC Microbiology·Jenny FernebroBirgitta Henriques Normark
May 20, 2008·Journal of Bacteriology·Julia FrunzkeMichael Bott
May 30, 2008·Journal of Clinical Microbiology·Yi-Jiun PanJin-Town Wang
Aug 5, 2008·Journal of Bacteriology·Julie ScottW Michael McShan
Jun 19, 2009·The Journal of Antimicrobial Chemotherapy·Carmen ArdanuyJosefina Liñares
Mar 12, 2011·The Journal of Antimicrobial Chemotherapy·Juyoun ShinKwan Soo Ko
Mar 15, 2011·The Journal of Infectious Diseases·Bernard W BeallAngela B Brueggemann
Aug 20, 2011·The Pediatric Infectious Disease Journal·Yu Chia HsiehTzou-Yien Lin
Jul 2, 2014·Infection and Immunity·Cuong Thach NguyenDong-Kwon Rhee
Jul 19, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Zitta Barrella HarboePalle Valentiner-Branth
Jan 20, 2018·Emerging Infectious Diseases·Satoshi NakanoSatoshi Ichiyama

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Citations

Aug 29, 2021·Journal of Biomedical Science·Yi-Yin ChenYu-Chia Hsieh
Dec 7, 2021·Frontiers in Cellular and Infection Microbiology·Antonio J Martín-Galiano, Ernesto García

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Datasets Mentioned

BETA
CP000921.1
CP001993.1

Methods Mentioned

BETA
PCR
Reverse Transcription Polymerase
lavages
RNA-seq

Software Mentioned

SPSS

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