PMID: 5903114Jan 1, 1966

Prophage repression as a model for the study of gene regulation. I. Titration of the lambda repressor

Journal of Bacteriology
H Wiesmeyer

Abstract

Wiesmeyer, Herbert (Vanderbilt University, Nashville, Tenn.). Prophage repression as a model for the study of gene regulation. I. Titration of the lambda repressor. J. Bacteriol. 91:89-94. 1966.-The concentration of lambda repressor molecules within a lambda lysogenic cell was estimated from the multiplicity of superinfecting homologous phage necessary to permit replication and release of plaque-forming units. A multiplicity of 20 superinfecting phage was found sufficient to permit replication to occur in the normal lambda lysogen. The phage released after lysis of the superinfected lysogen was composed of both prophage and superinfecting phage types. Superinfection of the lysogen at lower multiplicities resulted in the lysis of only a small percentage of infected cells and is thought to represent a possible heterogeneity of repressor concentration in the lysogenic population. Viability of the superinfecting particle was found to be unnecessary for titration of the repressor. The repressor concentration in three lysogens of the nonultraviolet-inducible mutant of lambda, lambda(ind-), was found to be greater than 20 regardless of the host bacterium. However, the number of cells yielding phage after superinfection was found to va...Continue Reading

Citations

Jan 1, 1969·Zeitschrift für allgemeine Mikrobiologie·D Noack, S Klaus
Jan 1, 1970·Zeitschrift für allgemeine Mikrobiologie·S Klaus
Jan 1, 1972·Molecular & General Genetics : MGG·A B Oppenheim, D Salomon
Jan 1, 1981·Molecular & General Genetics : MGG·M TheileE Geissler
Jan 1, 1971·Molecular & General Genetics : MGG·A B Oppenheim, Z Slonim

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Bacteriolysis
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