Prophagic DNA fragments in Streptococcus agalactiae strains and association with neonatal meningitis

Journal of Clinical Microbiology
Nathalie van der Mee-MarquetRoland Quentin

Abstract

We identified-by randomly amplified polymorphic DNA (RAPD) analysis at the population level followed by DNA differential display, cloning, and sequencing-three prophage DNA fragments (F5, F7, and F10) in Streptococcus agalactiae that displayed significant sequence similarity to the DNA of S. agalactiae and Streptococcus pyogenes. The F5 sequence aligned with a prophagic gene encoding the large subunit of a terminase, F7 aligned with a phage-associated cell wall hydrolase and a phage-associated lysin, and F10 aligned with a transcriptional regulator (ArpU family) and a phage-associated endonuclease. We first determined the prevalence of F5, F7, and F10 by PCR in a collection of 109 strains isolated in the 1980s and divided into two populations: one with a high risk of causing meningitis (HR group) and the other with a lower risk of causing meningitis (LR group). These fragments were significantly more prevalent in the HR group than in the LR group (P < 0.001). Our findings suggest that lysogeny has increased the ability of some S. agalactiae strains to invade the neonatal brain endothelium. We then determined the prevalence of F5, F7, and F10 by PCR in a collection of 40 strains recently isolated from neonatal meningitis cases f...Continue Reading

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Citations

Apr 1, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E van ElzakkerA van Belkum
Aug 31, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P Del GiudiceR Quentin
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Sep 7, 2006·Journal of Clinical Microbiology·Anne-Sophie DomelierRoland Quentin
Nov 17, 2011·MBio·Philippe LehoursAnders F Andersson
Sep 29, 2020·Frontiers in Microbiology·Chiara CrestaniRuth N Zadoks
Jun 14, 2019·Frontiers in Cellular and Infection Microbiology·Adélaïde RenardNathalie L van der Mee-Marquet
Aug 6, 2021·Frontiers in Microbiology·Clémence BeauruellePhilippe Lanotte

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