Prophylactic Dendritic Cell-Based Vaccines Efficiently Inhibit Metastases in Murine Metastatic Melanoma

PloS One
Oleg V MarkovMarina A Zenkova

Abstract

Recent data on the application of dendritic cells (DCs) as anti-tumor vaccines has shown their great potential in therapy and prophylaxis of cancer. Here we report on a comparison of two treatment schemes with DCs that display the models of prophylactic and therapeutic vaccination using three different experimental tumor models: namely, Krebs-2 adenocarcinoma (primary tumor), melanoma (B16, metastatic tumor without a primary node) and Lewis lung carcinoma (LLC, metastatic tumor with a primary node). Dendritic cells generated from bone marrow-derived DC precursors and loaded with lysate of tumor cells or transfected with the complexes of total tumor RNA with cationic liposomes were used for vaccination. Lipofectamine 2000 and liposomes consisting of helper lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine) and cationic lipid 2D3 (1,26-Bis(1,2-de-O-tetradecyl-rac-glycerol)-7,11,16,20-tetraazahexacosan tetrahydrocloride) were used for RNA transfection. It was shown that DCs loaded with tumor lysate were ineffective in contrast to tumor-derived RNA. Therapeutic vaccination with DCs loaded by lipoplexes RNA/Lipofectamine 2000 was the most efficient for treatment of non-metastatic Krebs-2, where a 1.9-fold tumor growth retard...Continue Reading

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Citations

May 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoying WangJun Dou
May 18, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sergey Vital'evich SennikovJulia Alexandrovna Shevchenko
Apr 23, 2017·Journal of Cancer Research and Clinical Oncology·Mariela A Moreno AyalaMarianela Candolfi
Mar 6, 2021·International Journal of Pharmaceutics·Farshad MirzaviMohammad Soukhtanloo

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Methods Mentioned

BETA
density gradient centrifugation
flow cytometry
PCR
transfection

Software Mentioned

STATISTICA

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