Prophylactic therapy with human amniotic fluid stem cells improved survival in a rat model of lipopolysaccharide-induced neonatal sepsis through immunomodulation via aggregates with peritoneal macrophages.

Stem Cell Research & Therapy
Yu SatoMamoru Tanaka

Abstract

Despite recent advances in neonatal care, sepsis remains a leading cause of mortality in neonates. Mesenchymal stem cells derived from various tissues, such as bone marrow, umbilical cord, and adipose tissue, have beneficial effects on adult sepsis. Although human amniotic fluid stem cells (hAFSCs) have mesenchymal stem cell properties, the efficacy of hAFSCs on neonatal sepsis is yet to be elucidated. This study aimed to investigate the therapeutic potential of hAFSCs on neonatal sepsis using a rat model of lipopolysaccharide (LPS)-induced sepsis. hAFSCs were isolated as CD117-positive cells from human amniotic fluid. Three-day-old rat pups were intraperitoneally treated with LPS to mimic neonatal sepsis. hAFSCs were administered either 3 h before or at 0, 3, or 24 h after LPS exposure. Serum inflammatory cytokine levels, gene expression profiles from spleens, and multiple organ damage were analyzed. hAFSC localization was determined in vivo. In vitro LPS stimulation tests were performed using neonatal rat peritoneal macrophages co-cultured with hAFSCs in a cell-cell contact-dependent/independent manner. Immunoregulation in the spleen was determined using a DNA microarray analysis. Prophylactic therapy with hAFSCs improved sur...Continue Reading

References

Jul 5, 2006·The Journal of Experimental Medicine·Shixin QinHuan Yang
Jul 11, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Anette M KunigSteven H Abman
Jan 9, 2007·Nature Biotechnology·Paolo De CoppiAnthony Atala
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Naveen GuptaMichael A Matthay
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jie ZhaoHong Tang
Sep 20, 2008·Nature Reviews. Immunology·Daniel RittirschPeter A Ward
Nov 18, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tobias R KollmannChristopher B Wilson
Jun 19, 2010·American Journal of Respiratory and Critical Care Medicine·Shirley H J MeiDuncan J Stewart
Jun 24, 2010·Clinics in Perinatology·James L Wynn, Ofer Levy
Jun 25, 2010·Cell Transplantation·Hiroshi YagiMartin L Yarmush
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Thomas J BartoshDarwin J Prockop
Jul 29, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Hiroshi YagiMartin L Yarmush
Apr 1, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mariuxi C ManukyanDaniel R Meldrum
Mar 28, 2013·Pediatric Pulmonology·Davide GrisafiPatrizia Zaramella
Nov 19, 2013·Immunity·Vincenzo Bronte, Mikael J Pittet
Mar 5, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lori F GentilePhilip A Efron
Oct 21, 2014·European Journal of Immunology·Thomas WinterbergJoachim F Kuebler
Mar 31, 2015·World Journal of Stem Cells·Eleuterio LombardoWilfried Dalemans
Oct 3, 2015·American Journal of Obstetrics and Gynecology·Chong Jai KimYeon Mee Kim
Jul 21, 2016·American Journal of Respiratory Cell and Molecular Biology·Heather L MendenVenkatesh Sampath
Nov 17, 2016·Brain, Behavior, and Immunity·Karla DrommelschmidtUrsula Felderhoff-Müser
Apr 25, 2017·Lancet·Andi L ShaneBarbara J Stoll
Sep 30, 2017·American Journal of Respiratory and Critical Care Medicine·Lauralyn A McIntyreUNKNOWN Canadian Critical Care Translational Biology Group
Feb 7, 2018·Scientific Reports·Michelangelo CorcelliPascale V Guillot
May 26, 2018·Pediatric Research·Kazumichi FujiokaDavid K Stevenson
Aug 4, 2018·Frontiers in Pediatrics·Simone S SchüllerOfer Levy
Nov 11, 2019·BMC Pulmonary Medicine·George CrowleyAnna Nolan
Nov 16, 2019·Stem Cell Research & Therapy·Jiaying LiuFuhua Yan

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Methods Mentioned

BETA
flow cytometry
enzyme-linked immunosorbent assay
lavage
ELISA
PCA
prophylactic therapy

Software Mentioned

ImageJ
JMP14
xPONENT®
Living Image

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