PMID: 8585093Sep 1, 1995Paper

Prophylaxis and treatment with a monoclonal antibody of tetrodotoxin poisoning in mice

Toxicon : Official Journal of the International Society on Toxinology
V R RiveraG S Bignami

Abstract

The ability of a tetrodotoxin (TTX)-specific monoclonal antibody to confer passive protection against lethal TTX challenge was investigated. The monoclonal antibody, T20G10, has an estimated affinity for TTX of approximately 10(-9) M and is about 50-fold less reactive with anhydrotetrodotoxin and unreactive with tetrodonic acid by competitive immunoassay. T20G10 specifically inhibited TTX binding in an in vitro radioligand receptor binding assay, but had no effect on the binding of saxitoxin to the sodium channel on rat brain membranes. In prophylaxis studies, mice were administered T20G10 via the tail vein 30 min prior to i.p. TTX challenge (10 micrograms/kg). Under these conditions, 100 micrograms T20G10 protected 6/6 mice, whereas 3/6 mice were protected with 50 micrograms T20G10. Non-specific control monoclonal antibody did not protect against lethality. Therapy studies simulating oral intoxication were performed with mice given a lethal dose of TTX by gavage in a suspension of non-fat dry milk in phosphate-buffered saline. Death occurred within 25-35 min in 6/6 mice not treated with T20G10. However, 500 micrograms T20G10 administered via the tail vein 10-15 min after oral TTX exposure prevented death in 6/6 mice. Lower dos...Continue Reading

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Citations

Dec 7, 2010·Transactions of the Royal Society of Tropical Medicine and Hygiene·Q T IslamD Mebs
Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Mark P KreuzerGeorge G Guilbault
Jul 1, 1997·Critical Care Clinics·D D Vernon, M C Gleich
Oct 24, 1998·Journal of Accident & Emergency Medicine·J Field
Oct 7, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Ulysses Paulino AlbuquerqueCecília de Fátima Castelo Almeida
Feb 26, 2014·Toxins·Vaishali BaneAmbrose Furey
May 23, 2014·Bioorganic & Medicinal Chemistry·Khalid Mohammed KhanMuhammad Iqbal Choudhary
Dec 26, 2013·Case Reports in Medicine·Y S YongA Ngo
Mar 23, 2010·Revista Do Instituto De Medicina Tropical De São Paulo·Claudia Carvalho Pestana SilvaVidal Haddad Junior

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