Propidium iodide staining: a new application in fluorescence microscopy for analysis of cytoarchitecture in adult and developing rodent brain

Micron : the International Research and Review Journal for Microscopy
Marcus HezelFaramarz Dehghani

Abstract

Immunohistochemical visualization of antigens in specimen has evolved to an indispensable technique in biomedical research for investigations of cell morphology and pathology both in bright field and fluorescence microscopy. While there are couple of staining methods that reveal entire cytoarchitecture in bright field microscopy such as Nissl or hemalaun-eosin, there are still limitations in visualizations of cytoarchitecture in fluorescence microscopy. The present study reports a simple staining method that provides the required illustration of cell allocations and cellular composition in fluorescence microscopy in adult and in developing rodent central nervous system using the fluorophore propidium iodide (PI, 5μg/mL). PI is a well-accepted marker for degenerating cells when applied prior to fixation (pre-fixation PI staining). Here, PI was added to the sections after the fixation (post-fixation PI staining). This revised labeling procedure led to similar cytoarchitectural staining patterns in fluorescence microscopy as observed with hemalaun in bright field microscopy. This finding was proven in organotypic hippocampal slice cultures (OHSC) and brain sections obtained from different postnatal developmental stages. Excitotoxi...Continue Reading

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Citations

Sep 12, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Xin-Yuan SunQiong-Zhi Gan
Mar 31, 2015·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·A AngelopoulouK Avgoustakis
May 6, 2016·Cell Adhesion & Migration·Tim HohmannFaramarz Dehghani
Dec 15, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kazuhide HayakawaEng H Lo
Sep 21, 2017·International Journal of Molecular Sciences·Tim HohmannFaramarz Dehghani
Aug 25, 2017·CNS Neuroscience & Therapeutics·Philipp PierohFaramarz Dehghani
May 1, 2020·Frontiers in Neuroscience·Ben LongXiangning Li
Feb 23, 2017·Pharmaceutical Research·Gabriella M Fernandes-CunhaFrancine Behar-Cohen
Jun 24, 2020·Chinese Journal of Integrative Medicine·Ashok MariDevaki Thiruvengadam
Dec 18, 2018·The Journal of Physical Chemistry. a·Thenahandi P D De SilvaIsiah M Warner

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