Propionate catabolism in Salmonella typhimurium LT2: two divergently transcribed units comprise the prp locus at 8.5 centisomes, prpR encodes a member of the sigma-54 family of activators, and the prpBCDE genes constitute an operon

Journal of Bacteriology
Alexander R Horswill, J C Escalante-Semerena

Abstract

We present the initial genetic and biochemical characterization of the propionate (prp) locus at 8.5 centisomes of the Salmonella typhimurium LT2 chromosome (T. A. Hammelman et al., FEMS Microbiol. Lett. 137: 233-239, 1996). In this paper, we report the nucleotide sequences of two divergently transcribed transcriptional units. One unit is comprised of the prpR gene (1,626 bp) encoding a member of the sigma-54 family of transcriptional activators; the second unit contains an operon of four genes designated prpB (888 bp), prpC (1,170 bp), prpD (1,452 bp), and prpE (1,923 bp). The heme biosynthetic gene hemB was shown by DNA sequencing to be located immediately downstream of the prpBCDE operon; hemB is divergently transcribed from prpBCDE and is separated from prpE by a 66-bp gap. In addition, we demonstrate the involvement of PrpB, PrpC, and PrpD in propionate catabolism by complementation analysis of mutants using plasmids carrying a single prp gene under the control of the arabinose-responsive P(BAD) promoter. Expression of prpB to high levels was deleterious to the growth of a prp+ strain on minimal medium supplemented with propionate as a carbon and energy source. We also report the cloning and overexpression of prpB, prpC, p...Continue Reading

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Citations

Oct 28, 1999·Nucleic Acids Research·H BarriosE Morett
Apr 9, 2005·Microbiology·Edith M SampsonThomas A Bobik
May 29, 2001·The Journal of Biological Chemistry·A R HorswillJ C Escalante-Semerena
Apr 13, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Sagar ChittoriM R N Murthy
Mar 21, 2006·Proteomics·Jens MattowUlrich E Schaible
Dec 22, 2016·Applied Microbiology and Biotechnology·Lukas MarschallChristoph Herwig
Feb 17, 2001·Molecular Biology of the Cell·C B EpsteinR A Butow
Mar 10, 2006·Applied Microbiology and Biotechnology·Thomas A Bobik
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Chiranjit ChowdhuryThomas A Bobik
Dec 1, 2010·Journal of Bacteriology·Tomohiro ShimadaAkira Ishihama
Dec 8, 2005·Biotechnology and Bioengineering·Sung Kuk Lee, Jay D Keasling
Apr 6, 2005·Molecular Genetics and Genomics : MGG·Torsten KoburgerM Hecker
Oct 22, 2016·Molecular Microbiology·Francesca M SimonteJohannes Gescher
Sep 11, 1999·Journal of Bacteriology·A R Horswill, J C Escalante-Semerena
Sep 1, 2005·Applied Microbiology and Biotechnology·Christian EweringAlexander Steinbüchel
Dec 2, 2009·Journal of Bacteriology·Christopher J Rocco, Jorge C Escalante-Semerena
Sep 3, 1998·Applied and Environmental Microbiology·Y M Kwon, S C Ricke
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·D M Downs, J C Escalante-Semerena
Mar 6, 1999·Microbiology and Molecular Biology Reviews : MMBR·L L Madison, G W Huisman

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