PMID: 9523824Apr 2, 1998Paper

Propofol and ketamine only inhibit intracellular Ca2+ transients and contraction in rat ventricular myocytes at supraclinical concentrations

Anesthesiology
Noriaki KanayaDerek S Damron

Abstract

The cellular mechanisms that mediate the cardiodepressant effects of intravenous anesthetic agents remain undefined. The objective of this study was to elucidate the direct effects of propofol and ketamine on cardiac excitation-contraction coupling by simultaneously measuring intracellular calcium concentration ([Ca2+]i) and shortening in individual, field-stimulated ventricular myocytes. Freshly isolated rat ventricular myocytes were loaded with the Ca2+ indicator, fura-2, and placed on the stage of an inverted fluorescence microscope in a temperature-regulated bath. [Ca2+]i and myocyte shortening (video edge detection) were monitored simultaneously in individual cells that were field-stimulated at 0.3 Hz. Baseline [Ca2+]i (mean +/- SEM) was 80 +/- 12 nM, and resting cell length was 112 +/- 2 microm. Field stimulation increased [Ca2+]i to 350 +/- 23 nM, and the myocytes shortened by 10% of diastolic cell length. Both intravenous anesthetic agents caused dose-dependent decreases in peak [Ca2+]i and shortening. At 300 microM, propofol prolonged time to peak concentration and time to 50% recovery for [Ca2+]i and shortening. In contrast, changes in time to peak concentration and time to 50% recovery in response to ketamine were ob...Continue Reading

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Citations

Oct 11, 2002·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Rie Kato, Pierre Foëx
Oct 9, 2008·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Noriko KamadaA Namiki
Feb 13, 2001·British Journal of Pharmacology·Jan van KlarenboschJacob J de Lange
Aug 18, 2000·Anaesthesia·I M Aguilera, R S Vaughan
Jul 27, 2000·Anesthesia and Analgesia·D L HamiltonP M Hopkins
Apr 23, 2003·Anesthesia and Analgesia·Sungsam ChoKoji Sumikawa
Feb 26, 2004·Anesthesia and Analgesia·A OuattaraB Riou
Feb 22, 2001·Journal of Molecular and Cellular Cardiology·L B StullC Schomisch Moravec
Dec 28, 2002·Anesthesiology·Noriaki KanayaA Namiki
Oct 27, 2011·Reproductive Toxicology·Jyotshnabala KanungoMerle G Paule
May 29, 2010·Korean journal of anesthesiology·Chul Ho ChangWyun Kon Park
Sep 2, 2006·Biochemical and Biophysical Research Communications·Shang-Jin KimJin-Shang Kim
Mar 3, 2007·Journal of Molecular and Cellular Cardiology·Barbara J McDermottRajinder K Mirakhur
Jan 20, 2011·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Assad HaneyaYork Zausig
Apr 26, 2002·Anesthesia and Analgesia·Noriaki KanayaA Namiki
Mar 26, 2003·European Journal of Anaesthesiology·T P WeberM Booke
Mar 26, 2003·European Journal of Anaesthesiology·P KrankeN Roewer
Mar 26, 2003·European Journal of Anaesthesiology·P BozkurtM Hacibekiroğlu
Mar 26, 2003·European Journal of Anaesthesiology·S G Sakka, A Meier-Hellmann
Mar 26, 2003·European Journal of Anaesthesiology·M SchywalskyH Schwilden
Mar 26, 2003·European Journal of Anaesthesiology·J A BlunkW Koppert
Feb 11, 2021·Chemical Research in Toxicology·Bonnie RobinsonJyotshna Kanungo

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