Propofol Enhances Hemoglobin-Induced Cytotoxicity in Neurons

Anesthesia and Analgesia
Jing YuanZhongcong Xie

Abstract

It has been increasingly suggested that propofol protects against hypoxic-/ischemic-induced neuronal injury. As evidenced by hemorrhage-induced stroke, hemorrhage into the brain may also cause brain damage. Whether propofol protects against hemorrhage-induced brain damage remains unknown. Therefore, in this study, we investigated the effects of propofol on hemoglobin-induced cytotoxicity in cultured mouse cortical neurons. Neurons were prepared from the cortex of embryonic 15-day-old mice. Hemoglobin was used to induce cytotoxicity in the neurons. The neurons were then treated with propofol for 4 hours. Cytotoxicity was determined by lactate dehydrogenase release assay. Caspase-3 activation was examined by Western blot analysis. Finally, the free radical scavenger U83836E was used to examine the potential involvement of oxidative stress in propofol's effects on hemoglobin-induced cytotoxicity. We found that treatment with hemoglobin induced cytotoxicity in the neurons. Propofol enhanced hemoglobin-induced cytotoxicity. Specifically, there was a significant difference in the amount of lactate dehydrogenase release between hemoglobin plus saline (19.84% ± 5.38%) and hemoglobin plus propofol (35.79% ± 4.41%) in mouse cortical neur...Continue Reading

References

Mar 12, 1999·British Journal of Clinical Pharmacology·J X Mazoit, K Samii
Jul 10, 2002·Stroke; a Journal of Cerebral Circulation·Xiaoying WangEng H Lo
Dec 24, 2004·Anesthesia and Analgesia·John R FeinerJennifer A Schuyler
Sep 27, 2007·CNS Drug Reviews·Chiara AdembriDomenico E Pellegrini-Giampietro
Mar 28, 2008·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Vincenzo Fodale, Enza La Monaca
Apr 2, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Flavio A LaraMarcus F Oliveira
Nov 28, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Glen C JicklingFrank R Sharp
Mar 13, 2014·Neurochemical Research·Song-sheng ShiXian-kun Tu
Jun 26, 2014·Neurochemical Research·Likui WangYuxian Shen
Sep 3, 2014·Brain Injury : [BI]·Deng-xing ZhangQi-feng Tang

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