Propofol facilitates excitatory inputs of cerebellar Purkinje cells by depressing molecular layer interneuron activity during sensory information processing in vivo in mice

Neuroreport
Yuan-Yuan HeDe-Lai Qiu

Abstract

Propofol is a rapid-acting sedative-hypnotic medication that has been widely used for the induction and maintenance of anesthesia; it has specific actions on different areas of the brain, such as sensory information transmission in the somatosensory cortex. However, the effects of propofol on the properties of sensory stimulation-evoked responses in cerebellar Purkinje cells (PCs) are currently unclear. In the present study, we studied the effects of propofol on facial stimulation-evoked responses in cerebellar PCs and molecular level interneurons (MLIs) in urethane-anesthetized mice using electrophysiological and pharmacological methods. Our results showed that cerebellar surface perfusion with propofol induced a decrease in the amplitude of the gamma-aminobutyric acid (GABA)-ergic component (P1) in a dose-dependent manner, but induced a significant increase in the amplitude of the excitatory response (N1). The IC50 of propofol-induced inhibition of P1 was 217.3 μM. In contrast, propofol (100 μM) depressed the spontaneous activity and tactile-evoked responses in MLIs. In addition, blocking GABA(A) receptor activity abolished the propofol (300 μM)-induced inhibition of the tactile-evoked inhibitory response and the increase in ...Continue Reading

References

Jan 1, 1994·Journal of Neurophysiology·E De Schutter, J M Bower
May 14, 2004·Journal of Neurophysiology·Mark C Bieda, M Bruce MacIver
Jul 24, 2004·Anesthesia and Analgesia·Akira KitamuraToshio Narahashi
Sep 15, 2004·Neuropsychobiology·Vladimir A FeshchenkoRuth A Reinsel
Aug 30, 2005·Trends in Pharmacological Sciences·Hugh C HemmingsNeil L Harrison
Aug 21, 2007·Nature Reviews. Neuroscience·Z J HuangF Ango
Nov 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Laura D LewisPatrick L Purdon

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Methods Mentioned

BETA
sedation

Software Mentioned

Clampex
Clampfit
SPSS

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