PMID: 16645449Apr 29, 2006Paper

Propofol-induced activation of protein kinase C isoforms in adult rat ventricular myocytes

Anesthesiology
Peter J WickleyDerek S Damron

Abstract

Myocardial protection by anesthetics is known to involve activation of protein kinase C (PKC). The authors' objective was to identify the PKC isoforms activated by propofol in rat ventricular myocytes. They also assessed the intracellular location of individual PKC isoforms before and after treatment with propofol. Freshly isolated ventricular myocytes were obtained from adult rat hearts. Immunoblot analysis of cardiomyocyte subcellular fractions was used to assess translocation of individual PKC isoforms before and after exposure to propofol. An enzyme-linked immunosorbent assay kit was used for measuring PKC activity. Immunocytochemistry and confocal microscopy were used to visualize the intracellular location of the individual PKC isoforms. Under baseline conditions, PKC-alpha, PKC-delta, and PKC-zeta were associated with both the cytosolic and membrane fractions, whereas PKC-epsilon was exclusively located in the cytosolic fraction. Propofol (10 microM) caused translocation of PKC-alpha, PKC-delta, PKC-epsilon, and PKC-zeta from cytosolic to membrane fraction and increased total PKC activity (211 +/- 17% of baseline; P = 0.003) in a dose-dependent manner. Immunocytochemical localization of the individual PKC isoforms demons...Continue Reading

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Citations

Oct 9, 2008·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Noriko KamadaAkiyoshi Namiki
Nov 19, 2011·Clinical and Experimental Pharmacology & Physiology·Yingxue SuBanghao Zhu
Mar 15, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Miomir JovicBato Korac
Mar 13, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Janet R ManningJo El J Schultz
Feb 28, 2007·Anesthesiology·Xueqin Ding, Paul A Murray
Jul 8, 2014·Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists·Long Wang, Wei-Feng Yu
Sep 22, 2009·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Michael WeissTobias Bednarek
Jul 7, 2009·Journal of Cardiothoracic and Vascular Anesthesia·Lukasz J KrzychAndrzej Bochenek
Oct 24, 2007·British Journal of Pharmacology·M-S SuleimanG D Angelini
Jun 11, 2009·Anesthesiology·Peter J WickleyDerek S Damron
Jul 11, 2007·Current Opinion in Anaesthesiology
Oct 16, 2021·American Journal of Physiology. Heart and Circulatory Physiology·Gaynor AguilarFabiola A Sánchez

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