Propofol induces MAPK/ERK cascade dependant expression of cFos and Egr-1 in rat hippocampal slices.

BMC Research Notes
Srivatsan KidambiJoseph Schianodicola

Abstract

Propofol is a commonly used intravenous anesthetic agent, which produce rapid induction of and recovery from general anesthesia. Numerous clinical studies reported that propofol can potentially cause amnesia and memory loss in human subjects. The underlying mechanism for this memory loss is unclear but may potentially be related to the induction of memory-associated genes such as c-Fos and Egr-1 by propofol. This study explored the effects of propofol on c-Fos and Egr-1 expression in rat hippocampal slices. Hippocampal brain slices were exposed to varying concentrations of propofol at multiple time intervals. The transcription of the immediate early genes, c-Fos and Egr-1, was quantified using quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). MAPK/ERK inhibitors were used to investigate the mechanism of action. We demonstrate that propofol induced the expression of c-Fos and Egr-1 within 30 and 60 min of exposure time. At 16.8 muM concentration, propofol induced a 110% increase in c-Fos transcription and 90% decrease in the transcription of Egr-1. However, at concentrations above 100 muM, propofol failed to induce expression of c-Fos but did completely inhibit the transcription of Egr-1. Propofol-induced c...Continue Reading

References

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Citations

Mar 1, 2012·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Jianguo HeLanhai Lv
Jun 15, 2013·Biomedical Microdevices·Evon S EreifejPamela J VandeVord
Oct 27, 2015·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Vesna PešićSabera Ruždijić
Aug 1, 2013·Biochemical and Biophysical Research Communications·Hyun Myung KoBon Nyeo Koo
Nov 9, 2016·Frontiers in Cellular Neuroscience·Weiguang WengThomas Behnisch
Apr 28, 2017·Hippocampus·Meghraj Singh Baghel, Mahendra Kumar Thakur
Mar 1, 2018·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Qing DuShilei Wang
Nov 18, 2015·Oncotarget·Ying JingGuanglei Zhuang

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Methods Mentioned

BETA
sedation

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