Propofol inhibits epileptiform activity in rat hippocampal slices

Epilepsy Research
P A RasmussenP A Rutecki

Abstract

Propofol (2,6 di-isopropylphenol) is an intravenous general anesthetic used widely in neuroanesthesia, as a sedative in intensive care units, and has successfully aborted refractory status epilepticus. We investigated the effects of propofol on epileptiform activity in rat hippocampal slices. Interictal epileptiform activity was produced by bath applying one of the following: picrotoxin (PTX; 10 and 50 microM), bicucculine methiodide (BMI; 10 and 50 microM), 4-aminopyridine (4-AP; 50 microM), 8.5 mM [K+]o or 0 [Mg2+]o artificial cerebrospinal fluid. Propofol was then added in increasing concentrations and the effect on the rate of extracellular field epileptiform discharges was measured. Ictal-like discharges (> 2 Hz for > 2 s) were produced by 7.5 mM [K+]o and pilocarpine (10 microM). Propofol (30 micrograms/ml, 168 microM) completely abolished discharges induced by 8.5 mM [K+]o and at 60 micrograms/ml (337 mM) completely suppressed discharges induced by 4-AP and 0 [Mg2+]o. Propofol was less effective in reducing discharges produced by GABAA/Cl- receptor complex antagonists. Propofol at a concentration of 300 micrograms/ml (1.7 mM) was needed to reduce BMI-induced (50 microM) discharges by 77% and only reduced PTX-induced (50 ...Continue Reading

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Citations

Aug 23, 2006·Der Nervenarzt·F BösebeckC Kellinghaus
Oct 3, 1999·Critical Care Medicine·J IgartuaM Sagy
Aug 8, 2009·Critical Care Medicine·Vivek N IyerAlejandro A Rabinstein
Jul 3, 2004·Epilepsia·Andrea O RossettiJulien Bogousslavsky
May 17, 2008·CNS Neuroscience & Therapeutics·Yoshinori KotaniHideaki Hara
May 20, 1999·The Veterinary Clinics of North America. Small Animal Practice·C E Short, A Bufalari
Apr 12, 2002·Epilepsy Research·Eldad J HadarPaul A Rutecki
Sep 24, 2004·Anesthesia and Analgesia·Hideya OhmoriAkiyoshi Namiki
Dec 18, 2008·Expert Review of Neurotherapeutics·Laura C Miller, Frank W Drislane
Sep 15, 2010·The Journal of Pharmacology and Experimental Therapeutics·Ashish DhirMichael A Rogawski
Apr 29, 1998·Current Opinion in Neurology·M C Walker
Jun 26, 1998·Journal of Neurophysiology·P A Rutecki, Y Yang

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