Propofol Promotes Activity and Tumor-Killing Ability of Natural Killer Cells in Peripheral Blood of Patients with Colon Cancer

Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Dongshui LiuMinmin Kong

Abstract

BACKGROUND We investigated the effect of propofol on activities and tumor-killing ability of natural killer (NK) cells in patients with colon cancer. MATERIAL AND METHODS Twenty colon cancer patients and 20 healthy subjects were included. Peripheral blood (5 ml) was collected from all patients and healthy subjects. NK cells in peripheral blood were separated by negative screening using immunomagnetic beads. Flow cytometry was used to determine expression of activated receptors, inhibitory receptors, killing effector molecules, and proliferation-associated markers on NK cell surfaces. After in vitro treatment with propofol for 24 h, expression of activated receptors, inhibitory receptors, killing effector molecules, and proliferation-associated markers on NK cell surfaces was examined again. In addition, the tumor-killing effect of NK cells was studied by co-culture with K562 cells or colon cancer SW620 cells at a ratio of 1: 1. RESULTS The number of NK cells in peripheral blood from colon cancer patients was increased compared with healthy subjects, but activities and proliferation ability of the NK cells were decreased. The tumor-killing effect of NK cells isolated from colon cancer patients was decreased. Of note, propofol pr...Continue Reading

Citations

Aug 30, 2019·Current Opinion in Anaesthesiology·Jan Rossaint, Alexander Zarbock
Aug 9, 2020·Local and Regional Anesthesia·Juan P CataMaria F Ramirez
Nov 19, 2020·Neuro-oncology Advances·Ann PrivorotskiyJuan P Cata
Oct 17, 2021·Fundamental & Clinical Pharmacology·Priyanka SahaDona Sinha

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Methods Mentioned

BETA
flow cytometry
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ModFit
SPSS

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