Abstract
Perioperative hyperglycemia is a common clinical metabolic disorder. Hyperglycemia could induce endothelial apoptosis and dysfunction. Propofol is a widely used IV anesthetic drug in clinical settings. In the present study, we examined whether and how propofol reduced high glucose-induced endothelial apoptosis and dysfunction in human umbilical vein endothelial cells (HUVECs). HUVECs were cultured with different concentrations (5, 10, 15, and 25 mM) of glucose for different times (4, 8, 12, and 24 hours). To study the effect of propofol, cells were incubated with different concentrations (0.2, 1, 5, and 25 μM) of propofol for 2 hours. In parallel experiments, cells were incubated in 5 mM glucose as control. Nitric oxide (NO) production was measured with a nitrate reductase assay. Cell viability was determined with a Cell Counting Kit-8. Protein expression of active caspase 3, cytochrome c, endothelial NO synthase (eNOS), p-eNOS-Thr, p66, protein kinase C βII (PKCβII), and p-PKCβII-Ser was measured by Western blot analysis. Accumulation of superoxide anion (O2˙) was measured with the reduction of ferricytochrome c. Cell apoptosis was determined with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling sta...Continue Reading
References
Nov 1, 1989·The Journal of Clinical Endocrinology and Metabolism·M R WerbE B Marliss
Aug 1, 1981·Anesthesiology·L F WaltsJ Brown
Jan 2, 1995·FEBS Letters·L AartsA Bast
Jan 12, 1999·Mediators of Inflammation·M Mathy-HartertM Lamy
Apr 28, 2000·Nature·T NishikawaM Brownlee
Feb 7, 2001·Circulation Research·U HinkT Munzel
Nov 28, 2001·Circulation·T HeitzerT Münzel
Jan 12, 2002·Circulation Research·Louis J IgnarroJoseph Loscalzo
Jan 17, 2002·The New England Journal of Medicine·G van den BergheR Bouillon
Mar 8, 2003·Hypertension·Sibel UlkerUlvi Bayraktutan
May 29, 2003·The Journal of Thoracic and Cardiovascular Surgery·Anthony P FurnaryAlbert Starr
Sep 25, 2003·Circulation·Mark A CreagerJoshua A Beckman
Mar 20, 2004·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Coen D A Stehouwer
Aug 11, 2004·Mayo Clinic Proceedings·James Stephen Krinsley
Feb 22, 2005·Cardiovascular Research·Shijie CaiKeith M Channon
Feb 3, 2006·The New England Journal of Medicine·Greet Van den BergheRoger Bouillon
May 9, 2006·Antioxidants & Redox Signaling·Enrica MigliaccioPier Giuseppe Pelicci
Feb 3, 2007·Science·Paolo PintonRosario Rizzuto
Dec 29, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Francesco CosentinoMassimo Volpe
Jan 29, 2008·Current Diabetes Reviews·Hironori NakagamiRyuichi Morishita
Feb 6, 2009·Anesthesiology·Angela K M Lipshutz, Michael A Gropper
Jul 19, 2011·Developmental Cell·Jean-Claude Martinou, Richard J Youle
Aug 6, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Yi ShiThomas F Lüscher
Dec 8, 2011·Antioxidants & Redox Signaling·Diane E Handy, Joseph Loscalzo
Dec 14, 2011·Anesthesia and Analgesia·Minmin ZhuJing Wang
Jun 14, 2012·Circulation Research·Francesco PaneniFrancesco Cosentino
Jan 15, 2013·Cardiovascular Diabetology·Minmin ZhuChanghong Miao
May 21, 2013·Surgery·Ruei-Ming ChenGong-Jhe Wu
Aug 31, 2013·Microvascular Research·Changfu LinYingchun Yang
Citations
Oct 21, 2015·Free Radical Research·Huijun ZhaoLingyi Kong
Jul 2, 2016·Journal of Translational Medicine·Dongdong YuanZiqing Hei
Jul 3, 2016·Fundamental & Clinical Pharmacology·Jiaqiang WangMinmin Zhu
Jul 31, 2016·Vascular Pharmacology·Weiwei QinFeihua Wu
Mar 29, 2014·European Journal of Pharmacology·José Ángel García-PedrazaAsunción Morán
Dec 10, 2016·Journal of Developmental Origins of Health and Disease·R Vidal-SantosV J Santana-Filho
Aug 15, 2017·Environmental Toxicology·Shu-Shong HsuWei-Zhe Liang
Apr 9, 2019·Autophagy·Xuan ZhaoJunying Miao
May 6, 2020·Acta Biochimica Et Biophysica Sinica·Xiangyuan ChenMinmin Zhu
Mar 1, 2017·BMC Anesthesiology·Yan LuChanghong Miao
May 26, 2017·PloS One·Yan LuChanghong Miao
Dec 28, 2018·Acta Biochimica Et Biophysica Sinica·Jing WangMinmin Zhu
May 17, 2019·Oxidative Medicine and Cellular Longevity·Jinjun PuJiang Hong
Sep 15, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Feng LiYanghao Guo