Propofol protects against high glucose-induced endothelial apoptosis and dysfunction in human umbilical vein endothelial cells

Anesthesia and Analgesia
Minmin ZhuChanghong Miao

Abstract

Perioperative hyperglycemia is a common clinical metabolic disorder. Hyperglycemia could induce endothelial apoptosis and dysfunction. Propofol is a widely used IV anesthetic drug in clinical settings. In the present study, we examined whether and how propofol reduced high glucose-induced endothelial apoptosis and dysfunction in human umbilical vein endothelial cells (HUVECs). HUVECs were cultured with different concentrations (5, 10, 15, and 25 mM) of glucose for different times (4, 8, 12, and 24 hours). To study the effect of propofol, cells were incubated with different concentrations (0.2, 1, 5, and 25 μM) of propofol for 2 hours. In parallel experiments, cells were incubated in 5 mM glucose as control. Nitric oxide (NO) production was measured with a nitrate reductase assay. Cell viability was determined with a Cell Counting Kit-8. Protein expression of active caspase 3, cytochrome c, endothelial NO synthase (eNOS), p-eNOS-Thr, p66, protein kinase C βII (PKCβII), and p-PKCβII-Ser was measured by Western blot analysis. Accumulation of superoxide anion (O2˙) was measured with the reduction of ferricytochrome c. Cell apoptosis was determined with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling sta...Continue Reading

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Jul 2, 2016·Journal of Translational Medicine·Dongdong YuanZiqing Hei
Dec 10, 2016·Journal of Developmental Origins of Health and Disease·R Vidal-SantosV J Santana-Filho
May 17, 2019·Oxidative Medicine and Cellular Longevity·Jinjun PuJiang Hong

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