Propolis modulates NOS2/arginase-1 pathway in tropomyosin-induced experimental autoimmune uveitis

Inflammopharmacology
Kahina TouriChafia Touil-Boukoffa

Abstract

In this study, we evaluated the preventive and curative effects of ethanolic extract of Propolis (EEP) during α-Tropomyosin-induced uveitis in an experimental model using Wistar rats, through the regulation of inducible nitric oxide synthase (NOS2) and arginase-1. In this context, rats received daily injection of EEP (100 mg/kg) for 5 days prior to immunization or for 9 days commencing 5 days post immunization with α-Tropomyosin extract, then were sacrificed at day 14. Histological examination, NOS2, arginase-1, and nuclear factor-κB (NF-κB) expression were evaluated in the retinas. Plasmatic production of nitric oxide (NO), urea, IL-4, and TNF-α was assessed. We have found that treatment with EEP substantially reduced the retinal histological damages induced by α-Tropomyosin. In the same context, a significant decrease of NO and TNF-α levels was noticed. Interestingly, EEP down-modulated NOS2 and NF-κB expression in retina. Also, an increase in urea and IL-4 levels was concomitant to an up-modulation of arginase-1 expression. Hence, it appears that EEP attenuated retinal damages through the induction of Th2 response and the inhibition of NF-κB/NOS2 pathway.

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Citations

Mar 25, 2021·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Mayara Rodrigues Brandão de PaivaSilvia Ligório Fialho
Sep 11, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Cibele Rodrigues ToledoArmando Silva-Cunha

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay

Software Mentioned

GraphPad
GraphPad Prism

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