PMID: 6990414Apr 1, 1980Paper

Proposed role of ATP in protein breakdown: conjugation of protein with multiple chains of the polypeptide of ATP-dependent proteolysis

Proceedings of the National Academy of Sciences of the United States of America
A HershkoI A Rose

Abstract

The heat-stable polypeptide ATP-dependent proteolysis factor 1 (APF-1) of the reticulocyte proteolytic system forms covalent compounds with proteins in an ATP-requiring reaction. APF-1 and lysozyme, a good substrate for ATP-dependent proteolysis, form multiple conjugates, as was shown by comigration of label from each upon gel electrophoresis. Multiple bands were also seen with other substrates of the ATP-dependent proteolytic system, such as globin or alpha-lactalbumin. Analysis of the ratio of APF-1 to lysozyme radioactivities and of the molecular weights of the bands indicated that they consist of increasing numbers of the APF-1 polypeptide bound to one molecule of lysozyme. The covalent linkage is probably of an isopeptide nature, because it is stable to hydroxylamine and alkali, and polylysine is able to give conjugates of APF-1. Removal of ATP after formation of the 125I-labeled APF-1 conjugates with endogenous proteins caused the regeneration of APF-1, indicating presence of an amidase. This reaction is thought to compete with proteases that may act on APF-1-protein conjugates, especially those containing several APF-1 ligands. A sequence of reactions in which the linkage of APF-1 to the substrate is followed by the prot...Continue Reading

References

Jan 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J D Etlinger, A L Goldberg
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·V Botbol, O A Scornik
Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·A HershkoI A Rose
Jun 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J W RobertsD W Mount
Apr 28, 1978·Biochemical and Biophysical Research Communications·A CiehanoverA Hershko
Jan 1, 1976·Annual Review of Biochemistry·A L Goldberg, A C St John
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·A CiechanoverA Hershko

❮ Previous
Next ❯

Citations

Jul 27, 2000·Journal of Cellular Physiology·S BusquetsJ M Argilés
Oct 1, 1991·Journal of Neuroscience Research·M NaashR E Anderson
Mar 27, 2002·Archives of Biochemistry and Biophysics·W David Strayhorn, Brian E Wadzinski
Jan 1, 1991·Acta Neuropathologica·B LachT R Davie
Apr 6, 2006·Journal of Biosciences·Dipankar NandiDilip Chandu
May 6, 2011·Cell Biochemistry and Biophysics·Arthur L Haas, Craig A Leach
May 23, 2013·Cell Biochemistry and Biophysics·Javier H JaraP Hande Özdinler
Jan 16, 2014·Indian Journal of Virology : an Official Organ of Indian Virological Society·Jeena KeezhedathSrinivas P Rao
Jun 1, 1991·Developmental Biology·M NemerA A Infante
Jan 1, 1981·Journal of Molecular and Cellular Cardiology·C J BeinlichH E Morgan
Dec 5, 1982·Journal of Molecular Biology·M F CharetteA Markovitz
Sep 1, 1989·Journal of the Neurological Sciences·D M MannM Landon
Mar 24, 1989·Cell·A Bachmair, A Varshavsky
Feb 10, 1988·Biochimica Et Biophysica Acta·S BeninatiJ E Folk
Dec 22, 1986·Biochimica Et Biophysica Acta·S M Rapoport, T Schewe
May 12, 2005·Experimental Cell Research·Jukka KallijärviAnna-Elina Lehesjoki
Jun 11, 1998·Mechanisms of Ageing and Development·M M ScrofanoA Taylor
Dec 23, 1998·Mechanisms of Ageing and Development·M M ScrofanoA Taylor
Sep 10, 1999·Postepy biochemii·M Staszczak, E Zdunek
Nov 1, 1996·Trends in Biochemical Sciences·A Hershko
Aug 2, 2005·Nature Cell Biology·Donald S KirkpatrickSteven P Gygi
Dec 3, 2005·Nature Reviews. Immunology·Yun-Cai LiuMichael Karin
Feb 3, 2005·Nature Reviews. Molecular Cell Biology·Aaron Ciechanover
Aug 14, 2008·Nature Reviews. Molecular Cell Biology·Tommer Ravid, Mark Hochstrasser
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A L Haas, I A Rose
Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A CiechanoverA Hershko
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C H Chung, A L Goldberg
Aug 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·L Waxman, A L Goldberg
Oct 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D T ChinM Rechsteiner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
A CiechanoverA Hershko
Proceedings of the National Academy of Sciences of the United States of America
J D Etlinger, A L Goldberg
Proceedings of the National Academy of Sciences of the United States of America
A HershkoH Heller
© 2021 Meta ULC. All rights reserved