PMID: 9657998Jul 11, 1998Paper

Proposed signal transduction role for conserved CheY residue Thr87, a member of the response regulator active-site quintet

Journal of Bacteriology
J L Appleby, Robert B Bourret

Abstract

CheY serves as a structural prototype for the response regulator proteins of two-component regulatory systems. Functional roles have previously been defined for four of the five highly conserved residues that form the response regulator active site, the exception being the hydroxy amino acid which corresponds to Thr87 in CheY. To investigate the contribution of Thr87 to signaling, we characterized, genetically and biochemically, several cheY mutants with amino acid substitutions at this position. The hydroxyl group appears to be necessary for effective chemotaxis, as a Thr-->Ser substitution was the only one of six tested which retained a Che+ swarm phenotype. Although nonchemotactic, cheY mutants with amino acid substitutions T87A and T87C could generate clockwise flagellar rotation either in the absence of CheZ, a protein that stimulates dephosphorylation of CheY, or when paired with a second site-activating mutation, Asp13-->Lys, demonstrating that a hydroxy amino acid at position 87 is not essential for activation of the flagellar switch. All purified mutant proteins examined phosphorylated efficiently from the CheA kinase in vitro but were impaired in autodephosphorylation. Thus, the mutant CheY proteins are phosphorylated...Continue Reading

References

Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R B BourretM I Simon
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A J Wolfe, H C Berg
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J D Liu, J S Parkinson
Jan 1, 1987·Methods in Enzymology·T A KunkelR A Zakour
Nov 1, 1994·Trends in Biochemical Sciences·R V SwansonM I Simon
Jun 4, 1993·Cell·J S Parkinson
Nov 9, 1993·Biochemistry·K Volz
May 1, 1993·Molecular Biology of the Cell·D BrayM I Simon
Sep 20, 1997·The Journal of Biological Chemistry·X ZhuP Matsumura
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·B E ScharfH C Berg

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Citations

Dec 14, 1999·Molecular Microbiology·J L Appleby, R B Bourret
Mar 30, 2001·The Journal of Biological Chemistry·S Y LeeD E Wemmer
May 17, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SchusterR B Bourret
May 10, 2000·The Journal of Biological Chemistry·M A Cervin, G B Spiegelman
Jan 21, 2004·Molecular Microbiology·Jenny G SmithRobert B Bourret
Jun 19, 2008·Molecular Microbiology·Stephanie A ThomasRobert B Bourret
Feb 15, 2014·Nature Communications·Anoop NarayananDinesh A Yernool
Aug 12, 2015·Journal of Bacteriology·TuAnh Ngoc HuynhValley Stewart
Nov 11, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alessandro PandiniJens Kleinjung
Nov 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Steven L PorterJudith P Armitage
Jun 19, 2008·Protein Science : a Publication of the Protein Society·Qiang Cui, Martin Karplus
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Jan 26, 2006·The Journal of Biological Chemistry·Elzbieta NowakPaul A Tucker
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Philippe RocheDaniel Kahn
Jul 31, 1998·Journal of Bacteriology·W N AbouhamadR B Bourret
Feb 9, 2005·Microbiology·Clara B García-CalderónFrancisco Ramos-Morales
Sep 7, 2020·Biophysical Journal·Paige WheatleyShahid Khan
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Tessa E F QuaxSonja-Verena Albers
Mar 25, 2000·Journal of Molecular Biology·R J LewisA J Wilkinson
Jan 29, 2002·Journal of Molecular Biology·Kirsten MattisonLinda J Kenney
Jun 6, 2000·Current Biology : CB·J Stock, S Da Re
Oct 20, 2006·Journal of Bacteriology·Collin M Dyer, Frederick W Dahlquist
Jun 26, 2007·Journal of Bacteriology·Elizabeth A HussaKaren L Visick
Jan 30, 2015·Biochemistry·Victor J OcasioKevin H Gardner
Jan 29, 2013·Journal of the American Chemical Society·Haihua LiangChuan He
Nov 9, 2007·Journal of Molecular Biology·Franziska F-F Schmid, Markus Meuwly

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