(Pro)renin receptor contributes to regulation of renal epithelial sodium channel

Journal of Hypertension
Syed Quadri, Helmy M Siragy

Abstract

Recent studies reported increased (Pro)renin receptor (PRR) expression during low-salt intake. We hypothesized that PRR plays a role in regulation of renal epithelial sodium channel (ENaC) through serum and glucocorticoid-inducible kinase isoform 1 (SGK-1)-neural precursor cell expressed, developmentally downregulated 4-2 (Nedd4-2) signaling pathway. Male Sprague-Dawley rats on normal-sodium diet and mouse renal inner medullary collecting duct cells treated with NaCl at 130  mmol/l (normal salt), or 63  mmol/l (low salt) were studied. PRR and α-ENaC expressions were evaluated 1 week after right uninephrectomy and left renal interstitial administration of 5% dextrose, scramble shRNA, or PRR shRNA (n = 6 each treatment). In-vivo PRR shRNA significantly reduced expressions of PRR throughout the kidney and α-ENaC subunits in the renal medulla. In inner medullary collecting duct cells, low salt or angiotensin II (Ang II) augmented the mRNA and protein expressions of PRR (P < 0.05), SGK-1 (P < 0.05), and α-ENaC (P < 0.05). Low salt or Ang II increased the phosphorylation of Nedd4-2. In cells treated with low salt or Ang II, PRR siRNA significantly downregulated the mRNA and protein expressions of PRR (P < 0.05), SGK-1 (P < 0.05), and...Continue Reading

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Jul 6, 2014·American Journal of Physiology. Renal Physiology·Syed Quadri, Helmy M Siragy

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Citations

Mar 10, 2016·Current Hypertension Reports·Nirupama Ramkumar, Donald E Kohan
Aug 16, 2016·International Journal of Molecular Sciences·Yiyun LouFan Jin
Apr 8, 2016·American Journal of Physiology. Renal Physiology·Nirupama RamkumarDonald E Kohan
Apr 29, 2016·American Journal of Physiology. Renal Physiology·Kexin PengTianxin Yang
May 24, 2018·American Journal of Physiology. Renal Physiology·Nirupama RamkumarDonald E Kohan
Mar 9, 2017·Journal of the American Society of Nephrology : JASN·Zhen LiYouhua Liu
Dec 17, 2019·American Journal of Physiology. Renal Physiology·Fei WangTianxin Yang
Jun 6, 2019·Nature Reviews. Nephrology·Atsuhiro Ichihara, Midori Sasaki Yatabe
Sep 30, 2016·American Journal of Physiology. Renal Physiology·Nirupama Ramkumar, Donald E Kohan
Jan 21, 2021·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Gertrude ArthurFrédérique B Yiannikouris
Dec 8, 2020·Frontiers in Physiology·Andrew C CurnowMinolfa C Prieto
Mar 30, 2021·Journal of Experimental Pharmacology·Syed S QuadriLudmila Nahar
Apr 8, 2021·Nature Reviews. Nephrology·Minolfa C PrietoL Gabriel Navar
Jun 24, 2021·The Journal of Pharmacology and Experimental Therapeutics·Manman QinJun Yu

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Methods Mentioned

BETA
ubiquitination
urine collections
transfection
light microscopy

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