Prosome cytodistribution relative to desmin and actin filaments in dividing C2.7 myoblasts and during myotube formation in vitro

Experimental Cell Research
F De ContoK Scherrer

Abstract

Prosomes constitute the multicatalytic proteinase (MCP) core of the 26S proteasomes, but were first observed as subcomplexes of untranslated mRNP; this suggests that they play a putative role in the control of protein biosynthesis in addition to their catabolic enzymatic function. In previous investigations it was shown that some prosomes colocalize with the intermediate filaments (IF) of the cytoskeleton, of the cytokeratin type in epithelial cells, and of the vimentin type in fibroblasts. Studies on adult rat muscle carried out with prosome-specific monoclonal antibodies (p-mAbs) have shown, surprisingly, that specific types of prosomes predominantly occupy a particular zone in between the M and the Z lines of the sarcomeric structure. The data presented here show that the subunit composition of prosomes changes when the dividing C2.7 myoblasts fuse into myotubes. We show furthermore that, in dividing C2.7 myoblasts, prosomes colocalize with the desmin network as well as with that of actin, in a distribution that changes with the subunit pattern of the prosomes investigated by individual p-mAbs. Surprisingly, when myogenic fusion is induced, specific types of prosomes move first to the nuclei; later on, they reappear in the c...Continue Reading

References

Nov 1, 1977·Cell·M Osborn, K Weber
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jun 4, 1992·Nature·A L Goldberg, K L Rock
Feb 1, 1992·Current Opinion in Cell Biology·R H Singer
Jan 1, 1991·Annual Review of Cell Biology·A Bretscher
Feb 20, 1986·Journal of Molecular Biology·C Martins de SaH P Schmid
Oct 1, 1988·The Journal of Cell Biology·M F Grossi de SaK Scherrer
Dec 5, 1988·FEBS Letters·W BaumeisterG Pfeifer
Nov 1, 1989·The Journal of Cell Biology·C LeeL B Chen
Jun 1, 1989·Molecular and Cellular Biology·C M de SaK Scherrer
Jan 1, 1986·Annual Review of Biochemistry·T D Pollard, J A Cooper
Jan 1, 1984·Cell and Muscle Motility·H R ByersK Fujiwara
Dec 1, 1981·The Journal of Cell Biology·I R Gibbons
Nov 1, 1981·The Journal of Cell Biology·A I GotliebV I Kalnins
Nov 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J W Mills, L J Mandel
Jan 1, 1994·Annual Review of Biochemistry·E Fuchs, K Weber
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·S D PatelH O McDevitt
Jun 1, 1993·Journal of Cellular Biochemistry·A B Fulton
Apr 1, 1993·Current Opinion in Genetics & Development·C P Emerson
Apr 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·L CripeA B Fulton
Sep 1, 1995·Current Biology : CB·K Barkalow, J H Hartwig
Jan 1, 1995·International Review of Cytology·P S Agutter
Oct 1, 1995·Kidney International·H F Cantiello
Sep 29, 1995·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T J Mitchison
Sep 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J V Small
Aug 1, 1996·The Journal of Cell Biology·R D GoldmanP M Steinert
Jan 1, 1996·Annual Review of Biochemistry·O CouxA L Goldberg

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Citations

Nov 25, 2004·Experimental Cell Research·Yann BassagliaJean Foucrier
May 8, 2001·Experimental Cell Research·J FoucrierK Scherrer
Feb 28, 2006·Biochemical and Biophysical Research Communications·Takeshi InoueKazuo Sutoh
Apr 2, 1998·Current Opinion in Immunology·A J Rivett
Apr 4, 2003·The International Journal of Biochemistry & Cell Biology·Cezary Wójcik, George N DeMartino

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