Prospect, isolation, and characterization of microorganisms for potential use in cases of oil bioremediation along the coast of Trindade Island, Brazil

Journal of Environmental Management
Edmo M RodriguesMarcos R Tótola

Abstract

In the present study, acrylic coupons with a thin layer of oil on the surface were incubated in the coastal water of Trindade Island, Brazil, for 60 days. The microorganisms adhered to the coupons were isolated using enrichment medium with hexadecane and naphthalene as the sole carbon and energy source. A total of 15 bacterial isolates were obtained, and the ability of these isolates to use different hydrocarbons as the source of carbon and energy was investigated. None of the isolates produced biosurfactants under our experimental conditions. Subsequently, identification methods such as partial sequencing of the 16S rRNA gene and analysis of fatty acids (MIDI) profile were employed. Among the 15 isolates, representatives of Actinobacteria, Firmicutes, and Alphaproteobacteria were detected. The isolates Rhodococcus rhodochrous TRN7 and Nocardia farcinica TRH1 were able to use all the hydrocarbons added to the culture medium (toluene, octane, xylene, naphthalene, phenanthrene, pyrene, hexadecane, anthracene, eicosane, tetracosane, triacontane, and pentacontane). Polymerase chain reaction amplification of the DNA isolated by employing primers for catechol 2,3-dioxygenase, alkane dehydrogenase and the alpha subunit of hydroxylatin...Continue Reading

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Citations

Nov 3, 2015·Marine Pollution Bulletin·Edmo M RodriguesMarcos R Tótola
Mar 5, 2016·Genome Announcements·Edmo M RodriguesMarcos R Tótola
Nov 30, 2018·Journal of Applied Microbiology·M Sánchez-GonzálezR Rojas-Herrera
Feb 23, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Edmo Montes RodriguesMarcos Rogério Tótola
Dec 27, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Edmo M RodriguesMarcos R Tótola
Aug 9, 2019·Environmental Science and Pollution Research International·Urooj Naeem, Muhammad Akram Qazi
Apr 5, 2021·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Celia Marcela Camacho-MontealegreMarcos Rogério Tótola

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