Prospective comparison of direct immunofluorescence and conventional staining methods for detection of Giardia and Cryptosporidium spp. in human fecal specimens.

Journal of Clinical Microbiology
A J AllesA R Mattia

Abstract

In a prospective comparative study, 2,696 consecutive fresh stool specimens over the course of 1 year were examined for Giardia lamblia and Cryptosporidium parvum by using a direct immunofluorescent-monoclonal antibody stain (for unspun specimens) and conventional staining methods (chlorazol black E for Giardia cysts and modified Kinyoun acid-fast for Cryptosporidium oocysts). The direct immunofluorescent-monoclonal antibody method resulted in a significantly increased detection rate for both giardia (118 versus 79 specimens, 49.4%; P = 0.006) and cryptosporidia (39 versus 23 specimens, 69.6%; P = 0.055).

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Citations

Dec 15, 2011·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Weslen Fabricio Pires TeixeiraMarcelo Vasconcelos Meireles
Sep 2, 2016·Journal of the American Veterinary Medical Association·Meriam N SalehAnne M Zajac
Sep 1, 2016·Journal of Clinical Microbiology·Alessandro Rossi, Marc Roger Couturier
Dec 1, 2006·Annals of the New York Academy of Sciences·Sathaporn JittapalapongRoger W Stich
Feb 26, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Beauty E OmoruyiFrancis O Okonkwo
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Jun 23, 2015·Current Opinion in Infectious Diseases·Michael Angarone, Michael G Ison
Nov 17, 2017·Clinical Microbiology Reviews·Lynne S GarciaGovinda Visvesvara
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Nov 8, 2013·Journal of Clinical Microbiology·Ian H McHardyRomney M Humphries
Jul 13, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·Maria das Graças Cabral PereiraAlverne Passos Barbosa
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Jun 20, 2018·Veterinary World·Natapol Pumipuntu, Supawadee Piratae
Apr 15, 2019·Journal of Veterinary Internal Medicine·Meriam N SalehAnne M Zajac
Jul 30, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Meriam N SalehAnne M Zajac
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Oct 16, 2009·Journal of Clinical Microbiology·David J JavierRebecca Richards-Kortum
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Apr 27, 2000·Archives of Pathology & Laboratory Medicine·B S Reisner, J Spring

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