Prospective comparison of two flow cytometry methodologies for monitoring minimal residual disease in a multicenter treatment protocol of childhood acute lymphoblastic leukemia

Cytometry. Part B, Clinical Cytometry
Drorit LuriaIsrael National Study Group of Childhood ALL

Abstract

Minimal residual disease (MRD) is a powerful prognostic indicator in childhood acute lymphoblastic leukemia (ALL). Multiparametric flow cytometry (FC) is a rapid and sensitive methodology for detection of MRD, applicable for most patients and is being incorporated in multicenter treatment protocols. The influence of different techniques and of individual interpretation of data on the interlaboratory variability in FC-MRD determinations has not been described. We compared FC-MRD of identical bone marrow samples processed as either Ficoll separated mononuclear cells or lyse and wash nucleated cells (NC) in two central laboratories of a national multicenter childhood ALL study. A total of 290 samples at diagnosis and 494 follow-up samples (Day-15 n = 261; Day-33 n = 233) were analyzed. A group of 52 paired list mode data (LMD) of D-15 and D-33 samples was blindly reanalyzed by both laboratories. Pearson correlations for all samples of D-15 (n = 261) and D-33 (n = 233) were 0.875 and 0.82, respectively (P < 0.001), being lower for T-ALL 0.716 and 0.719, respectively. Quantitative concordance defined as less than 0.5 log difference in MRD measured by the two methodologies was 80.8% at D-15 but only in 57.9% at D-33. Reanalysis of LM...Continue Reading

References

Dec 16, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J CiudadA Orfao
Sep 29, 1999·Statistical Methods in Medical Research·J M Bland, D G Altman
Mar 6, 2002·Blood·Michael N DworzakUNKNOWN Austrian Berlin-Frankfurt-Münster Study Group
May 4, 2002·Best Practice & Research. Clinical Haematology·Dario Campana, Elaine Coustan-Smith
Oct 21, 2003·Leukemia & Lymphoma·Michael N Dworzak, Eva R Panzer-Grümayer
Sep 18, 2009·Pediatric Blood & Cancer·Ester MejstríkováOndrej Hrusák
Oct 7, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Giuseppe BassoGiuseppe Gaipa

❮ Previous
Next ❯

Citations

Mar 1, 2012·Current Hematologic Malignancy Reports·Dario Campana
Apr 25, 2012·Current Opinion in Hematology·Dario Campana
May 15, 2015·Cytometry. Part B, Clinical Cytometry·Joseph A DiGiuseppeDana Pe'er
Jan 26, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·S ChirumboloAntonio Vella
Jun 14, 2017·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Luca Basso-RicciLuca Biasco
Jan 16, 2013·The Korean Journal of Hematology·Dario Campana, Elaine Coustan-Smith
Mar 24, 2016·Cancer Biomarkers : Section a of Disease Markers·Azza A Aboul EneinLamiaa A Fathalla
Aug 22, 2017·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·Gaurav ChatterjeePrashant R Tembhare
Apr 7, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shifra AshSmadar Avigad

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.