Prospective evaluation of pyrosequencing for the rapid detection of isoniazid and rifampin resistance in clinical Mycobacterium tuberculosis isolates.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
H J MarttilaH Soini

Abstract

A pyrosequencing-based method for the rapid detection of isoniazid (INH) and rifampin (RIF) resistance in Mycobacterium tuberculosis was evaluated in clinical practice. The method can detect the INH resistance-causing katG315 mutation, and all mutations in the RIF resistance-determining rpoB core region, in less than 6 h from cultured isolates. The method was first validated with 42 isolates, and was subsequently prospectively evaluated with 91 isolates, including clinical isolates and external quality control assessment strains, over a period of 2.5 years. The pyrosequencing results of clinical isolates were available, on average, 19 days earlier (median 19 days; range 3-43 days) than conventional susceptibility testing results. The composite data showed that the sensitivity of pyrosequencing for detecting resistance correctly was 66.7% for INH and 97.4% for RIF. The specificity of pyrosequencing was 100% for both drugs. Acceptable sensitivity for detecting resistance and the rapidness of pyrosequencing make it a valuable tool in the clinical setting.

References

Oct 1, 1995·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·H De BeenhouwerF Portaels
Oct 15, 1996·FEMS Microbiology Letters·H TaniguchiS Yoshida
Aug 15, 1998·Science·M RonaghiP Nyrén
May 22, 2003·Tuberculosis·Marcos A Espinal
May 23, 2003·Antimicrobial Agents and Chemotherapy·Daiva BakonytePetras Stakenas
Sep 25, 2003·International Journal of Medical Microbiology : IJMM·Maria KolakJon Jonasson
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·Rosilene Fressatti CardosoMario H Hirata
Oct 13, 2004·Molecular Biotechnology·Matthew A Diggle, Stuart C Clarke
Nov 6, 2004·Journal of Clinical Microbiology·Takayuki WadaKazuo Kobayashi
Feb 1, 2005·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C ArnoldS Gharbia
Feb 9, 2005·Diagnostic Microbiology and Infectious Disease·Jin Rong ZhaoXiao Jun Yan
Apr 13, 2005·Journal of Microbiological Methods·Daniela IsolaUNKNOWN LONG-DRUG study group
Jun 9, 2005·JAMA : the Journal of the American Medical Association·Christopher DyeMario C Raviglione
Jun 8, 2006·Journal of Clinical Microbiology·Pontus JureenSven E Hoffner
Jul 28, 2006·Antimicrobial Agents and Chemotherapy·Manzour Hernando HazbónDavid Alland

❮ Previous
Next ❯

Citations

Oct 23, 2009·Journal of Clinical Microbiology·Lulette Tricia C BravoNabin K Shrestha
Dec 3, 2009·BMC Research Notes·Zoheira DjelouadjiMichel Drancourt
Aug 14, 2012·The American Journal of the Medical Sciences·Ameeta S KalokheMinh Ly T Nguyen
May 27, 2014·Clinics in Laboratory Medicine·Shou-Yean Grace Lin, Edward P Desmond
Dec 7, 2010·Journal of Microbiological Methods·Gemeda AbebeRobert Colebunders
Aug 8, 2009·Respiratory Medicine·Suhail Ahmad, Eiman Mokaddas
Sep 1, 2016·BMC Infectious Diseases·Sophia B GeorghiouTimothy C Rodwell
Jul 20, 2021·Journal of Clinical Tuberculosis and Other Mycobacterial Diseases·Emnet GetachewTsegahun Manyazewal

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
E Garza-GonzálezV Bocanegra-García
© 2022 Meta ULC. All rights reserved