Prospective evaluation of rapid antimicrobial susceptibility testing by disk diffusion on Mueller-Hinton rapid-SIR directly on blood cultures

Diagnostic Microbiology and Infectious Disease
Claire PérillaudJean-Claude Nguyen Van

Abstract

With the worldwide spread of antibiotic resistance, delivering antibiotic susceptibility test (AST) results in a timely manner represents a major challenge. In cases of sepsis, rapid AST may facilitate early optimization of empiric antibiotic therapy. Disc diffusion is a well-standardized AST method, however 16 to 24 h are required to achieve an overall AST profile according to antimicrobial societies. In this prospective pilot study, we evaluated the performance of Mueller-Hinton-Rapid-SIR (MHR-SIR) agar after 6-8 h of incubation in comparison with standard MH agar after 16 h of incubation directly on positive blood cultures caused by Enterobacteriaceae and Staphylococcus aureus from routine clinical microbiology. A total of 133 positive blood samples including 110 Enterobacteriaceae (83%) and 23 Staphylococcus aureus (17%) were tested in parallel by two direct AST methods, each using EUCAST breakpoints. For each combination bacterium and antibiotic, we compared the categorical agreement and the correlation between the diameters obtained by MHR-SIR and by standard MH. Our results showed 97.7% categorical agreement for Enterobacteriaceae, with 1.4% minor errors, 0.4% major errors and 0.5% very major errors. For S. aureus, we ob...Continue Reading

Associated Clinical Trials

Citations

Aug 5, 2019·The Journal of Antimicrobial Chemotherapy·Benoît PilmisJean-Claude Nguyen Van
Feb 6, 2020·The Journal of Antimicrobial Chemotherapy·Emma JonassonGunnar Kahlmeter
Aug 23, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Gabriele BiancoCristina Costa
Nov 14, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Merav StraussRaul Colodner
Aug 14, 2020·The Journal of Antimicrobial Chemotherapy·Anna ÅkerlundUNKNOWN RAST Study Group
Apr 4, 2019·World Journal of Microbiology & Biotechnology·Emilio Cendejas-BuenoJesús Mingorance
May 22, 2020·Journal of Clinical Microbiology·Catherine Anne HoganNiaz Banaei
Oct 5, 2020·Infectious Disease Clinics of North America·Andrea EndimianiMichael R Jacobs

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