Prospective identification and purification of hair cell and supporting cell progenitors from the embryonic cochlea.

Brain Research
Angelika DoetzlhoferNeil Segil

Abstract

Expression of the cyclin-dependent kinase inhibitor p27(Kip1) defines a post-mitotic population of cells in the embryonic mammalian cochlea that constitutes the nascent organ of Corti. Here, we describe techniques to purify these precursors using a transgenic p27/GFP reporter and fluorescence activated cell sorting (FACS). We demonstrate that these cells express other markers of the sensory lineage, such as Sox2, and when placed in dissociated cell culture differentiate as hair cells and supporting cells. The purified sensory progenitors thus obtained provide a means of studying the process of hair cell and supporting cell differentiation in vitro, as well as providing a means of analyzing the molecular and physiological properties of this unique population of cells.

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Citations

Apr 14, 2010·Journal of the Association for Research in Otolaryngology : JARO·Ronna HertzanoScott E Strome
Nov 23, 2010·Journal of Translational Medicine·Jeanne OiticicaRicardo F Bento
Jul 26, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Sheng-zhi WangLi I Zhang
Jan 21, 2016·Scientific Reports·Dean P CampbellAngelika Doetzlhofer
Jun 9, 2015·Journal of Anatomy·Martin L BaschAndrew K Groves
Jan 15, 2011·Progress in Neurobiology·Jessica M Appler, Lisa V Goodrich
May 31, 2007·The Journal of Comparative Neurology·Joana NevesFernando Giraldez
Dec 24, 2014·Cell and Tissue Research·Robert Durruthy-Durruthy, Stefan Heller
Aug 17, 2016·Frontiers of Medicine·Muhammad WaqasRenjie Chai
Dec 4, 2019·Stem Cell Research & Therapy·Cheng ChengRenjie Chai
Aug 23, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xiao-Jun Li, Angelika Doetzlhofer
Sep 1, 2020·Hearing Research·Michael Ebeid, Sung-Ho Huh
Mar 27, 2021·Stem Cell Reports·Dunia Abdul-AzizAlbert S B Edge

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