Prospective simultaneous quantification of human cytomegalovirus-specific CD4+ and CD8+ T-cell reconstitution in young recipients of allogeneic hematopoietic stem cell transplants

Blood
Daniele LilleriFranco Locatelli

Abstract

We investigated immune reconstitution against human cytomegalovirus (HCMV) in 57 hematopoietic stem cell transplant (HSCT) recipients, aged 1 to 24 years, through a novel method combining T-cell stimulation by HCMV-infected autologous dendritic cells with simultaneous cytometric quantification of HCMV-specific, IFNgamma-producing CD4(+) and CD8(+) T cells. Lymphoproliferative response (LPR) to HCMV antigens was also determined. Patients were stratified into 2 groups according to HCMV serostatus, comprising 39 HCMV-seropositive (R(+)) and 18 HCMV-seronegative (R(-)) patients who received a transplant from a sero-positive donor. Recovery of both HCMV-specific CD4(+) and CD8(+) T-cell immunity occurred in all 39 R(+) patients within 6 months and in 6 (33%) of 18 R(-) patients within 12 months. In R(+) patients, the median numbers of HCMV-specific CD8(+) and CD4(+)T cells were significantly higher than those of healthy controls, starting from days +60 and +180, respectively. In (R-) patients, the median numbers of HCMV-specific T cells were consistently lower than in R(+) patients. LPR was delayed compared with reconstitution of IFNgamma-producing T cells. Patients with delayed specific immune reconstitution experienced recurrent e...Continue Reading

References

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Citations

Jul 2, 2008·The Journal of Infectious Diseases·Daniele LilleriGiuseppe Gerna
Jul 5, 2007·Current Opinion in Infectious Diseases·W Lawrence Drew
Jan 1, 2014·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Adriana WeinbergMark A Jacobson
Dec 18, 2013·Diagnostic Microbiology and Infectious Disease·Sylvia BorchersEva M Weissinger
Aug 9, 2011·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Emilia GeniniGiuseppe Gerna
Jan 22, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Fausto BaldantiGiuseppe Gerna
Aug 12, 2015·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Elisa GabantiGiuseppe Gerna
Jul 19, 2011·Pediatric Transplantation·Manisha PatelBetsy C Herold
Jan 20, 2010·Reviews in Medical Virology·M Grazia Revello, Giuseppe Gerna
Jul 16, 2015·Cellular and Molecular Life Sciences : CMLS·Miriam CiáurrizNatalia Ramírez
Jul 30, 2014·Transplant International : Official Journal of the European Society for Organ Transplantation·Ildefonso EspigadoPilar Perez-Romero
May 28, 2014·Journal of Immunology Research·Xiao-Hua LuoXiao-Jun Huang
Nov 1, 2016·Expert Review of Hematology·Franco LocatelliPietro Merli
Apr 14, 2018·European Journal of Haematology·Thomas WidmannMartina Sester

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