Prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time PCR

Journal of Clinical Microbiology
Marie L LandryDavid Ferguson

Abstract

A Light Diagnostics human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA), and the results were compared to those for TaqMan reverse transcription-PCR (RT-PCR). Of the 202 samples tested, 48 were positive by RT-PCR, and 41 (85.4%) of these were positive by DFA (P = 0.0771). The commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.

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Citations

Feb 5, 2009·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·J-K ChunD S Kim
Mar 20, 2009·The Pediatric Infectious Disease Journal·Rolando Ulloa-GutierrezWilbert Alfaro-Bourrouet
Oct 7, 2011·Clinical Microbiology Reviews·Verena SchildgenOliver Schildgen
Jun 6, 2009·Future Microbiology·Elena R VirtueLin-Fa Wang
Aug 9, 2011·Expert Review of Anti-infective Therapy·Sigvard OlofssonMagnus Lindh
Aug 1, 2008·Expert Opinion on Medical Diagnostics·Katia Jaton-Ogay, Jacques Bille
May 18, 2010·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Christina SchinderaMaria Teresa Barbani
Nov 7, 2009·Clinics in Laboratory Medicine·Marie Louise Landry
Nov 18, 2008·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Irena Kukavica-Ibrulj, Guy Boivin
Jun 9, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Maria Teresa Barbani, Meri Gorgievski-Hrisoho
Feb 17, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P JokelaM Lappalainen
Dec 18, 2015·Revista Da Sociedade Brasileira De Medicina Tropical·Jonas Michel WolfVagner Ricardo Lunge
Aug 16, 2019·Journal of Clinical Microbiology·Yi-Wei Tang
Jun 19, 2015·Clinical Microbiology Reviews·Jose L SanchezCharlotte A Gaydos

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